traT and CNF2 genes of Escherichia coli isolated from milk of healthy cows and sheep |
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| Authors: | Açik M N Yurdakul N E Cakici L Onat N Dogan O Cetinkaya B |
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| Institution: | Department of Microbiology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey. |
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| Abstract: | The objectives of the present study were to isolate Escherichia coli from milk of apparently healthy cows and sheep and to investigate the presence of traT and cytotoxic necrotising factor-2 (CNF2) virulence genes by multiplex polymerase chain reaction (PCR). Milk samples collected from a total of 1028 apparently healthy ruminants (737 cows and 291 sheep) in eastern Turkey were streaked onto 5% sheep-blood agar. E. coli was isolated and identified by biochemical tests in 5.9% (61/1028) of milk samples. Correct amplification with the molecular length of 232 bp was obtained from all E. coli isolates by the species-specific PCR. The isolation rates of the agent were calculated to be 7.6% (56/737) in cows and 1.7% (5/291) in sheep. The difference between these proportions was statistically significant (p < 0.001). Multiplex PCR showed that traT and CNF2 genes were present in 62.3% and 6.6% of all isolates, respectively. Both genes were present in 16.4% of the isolates, with only 14.7% having neither gene. |
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| Keywords: | E coli traT CNF2 Milk Cow Sheep |
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