| 牛卵母细胞显微操作机械去核方法的比较 |
| |
| 引用本文： | 姜成国,林涛,尹多,李钟淑,方南洙.牛卵母细胞显微操作机械去核方法的比较[J].延边大学农学学报,2009,31(4):238-242. |
| |
| 作者姓名： | 姜成国 林涛 尹多 李钟淑 方南洙 |
| |
| 作者单位： | 1. 延边州畜牧总站,吉林,延吉,133000
2. 延边大学农学院,吉林,龙井,133400 |
| |
| 基金项目： | 国家自然科学基金项目，振兴东北老工业基地项目，州校合作项目 |
| |
| 摘 要： | 分别采用挤压去核法、盲吸去核法和点击去核法对延边黄牛卵母细胞进行去核操作,在操作时间、成功率、去核率及重组胚后期发育等方面进行比较.结果表明,去核率上,盲吸法低于其他2种方法;去核成功率上,挤压法和点压法高于盲吸法;操作耗时上,挤压法和盲吸法耗时较短;3种方法所构建的重组胚卵裂率以及囊胚发育率均差异不显著.因此,挤压去核法是一种较适合延边黄牛卵母细胞去核的有效方法.
|
| 关 键 词： | 卵母细胞 去核方法 核移植 |
Comparison of micromanipulation mechanical enucleation methods of bovine oocytes |
| |
| JIANG Cheng-guo,LIN Tao,YIN Duo,LI Zhong-shu,FANG Nan-zhu.Comparison of micromanipulation mechanical enucleation methods of bovine oocytes[J].Journal of Agricultural Science Yanbian University,2009,31(4):238-242. |
| |
| Authors: | JIANG Cheng-guo LIN Tao YIN Duo LI Zhong-shu FANG Nan-zhu |
| |
| Institution: | JIANG Cheng-guo , LIN Tao, YIN Duo, LI Zhong-shu, FANG Nan-zhu ( 1. Animal Husbandry Central of Yanbian State ,Yanji Jilin 133000,China; 2. Agricultural College of Yanbian University ,Longjing Jilin ,133400,China) |
| |
| Abstract: | Oocytes of Yanbian Yellow Cattle were enucleated by methods of squeezing enucleation, blind enucleation and point-hitting enucleation, then compared in operating time, achievement rate, enucleation rate and proportion of reconstituted embryos . The results showed that blind enucleation was lower than the other two ways in enucleation rate. Higher success rate were obtained using squeezing enucleation and point-hitting enucleation. The shorter time-consuming methods were squeezing enucleation and blind enucleation; there is no significant difference in cleavage rate and blastocyst rate. Therefore, the methods of squeezing enucleation was a practical enucleation method of Yanbian Yellow Cattle oocytes. |
| |
| Keywords: | oocyte method of enucleation nuclear transplantation |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
