The development of an international reference panel of monoclonal antibodies for the differentiation of hog cholera virus from other pestiviruses. |
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| Authors: | S Edwards V Moennig G Wensvoort |
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| Institution: | Central Veterinary Laboratory, Ministry of Agriculture Fisheries and Food, New Haw, Weybridge, Surrey, UK. |
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| Abstract: | A panel of 30 monoclonal antibodies was defined and characterized with respect to the binding capacity in immunoperoxidase assay to different strains of pestivirus. Using the panel it was possible to identify specifically all strains and isolates of hog cholera virus, hog cholera vaccines derived from 'C' strains, and most strains of bovine viral diarrhoea/border disease (BVD/BD) viruses (including those isolated from pigs). A small proportion of BVD/BD isolates from pigs and ruminants reacted only with the monoclonals specific for pestivirus group antigen. It is recommended that monoclonal typing methods be introduced into official procedures for the diagnosis of hog cholera/classical swine fever. |
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