| 二氯喹啉酸·苄嘧磺隆复合污染降解菌的特性研究 |
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| 作者姓名： | L&#; Zhen-Mei LI Zi-Mu SANG Li-Y MIN Hang |
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| 作者单位： | College of Resources and Environment, Southwest University, Chongqing 400716 （China）. |
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| 基金项目： | 国家重点科技研究发展项目，重庆市自然科学基金 |
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| 摘 要： | A bacterial strain, designated as LS, was isolated from a contaminated soil and was found to be capable of utilizing quinclorac, bensulfuronmethyl, and a mixture of the two as carbon and energy sources for growth. Strain LS was identified as Ochrobactrum sp. based on its physiological-biochemical properties, 16S rDNA sequences, and phylogenetic analysis. The extent of degradation of quinclorac and bensulfuronmethyl at initial concentrations of 1.5 and 0.1 g L^-1 was 90% and 67%, respectively, as measured by high performance liquid chromatography （HPLC）. When a herbicide mixture of 0.34 g L^-1 quinclorac and 0.02 g L^-1 bensulfuronmethyl was applied as carbon sources, quinclorac and bensulfuronmethyl were degraded at 95.7% and 67.5%, respectively. It appears that quinclorac is utilized more easily in a mixture than in a single state. The optimal temperature for growth of strain LS was 37 ℃. Strain LS grew well at pH 6 to 9 and had the highest degradation level for quinclorac and bensulfuronmethyl at an initial pH of 7 and 8, respectively. Addition of 0.25 g L^-1 yeast extract could promote the growth and extent of degradation of quinclorac and bensulfuronmethyl by strain LS. Strain LS also showed the capability to degrade other aromatic compounds such as catechol, propisochlor, 4-chloro-2-methylphenoxyacetic acid sodium （MCPA-Na） and imazethapy. The isolate LS shows a huge potential to be used in bioremediation for treating complex herbicide residues.
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| 关 键 词： | 除草剂 生物降解 菌株 土壤污染 |
Characterization of a strain capable of degrading a herbicide mixture of quinclorac and bensulfuronmethy |
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| L&#; Zhen-Mei,LI Zi-Mu,SANG Li-Y,MIN Hang.Characterization of a strain capable of degrading a herbicide mixture of quinclorac and bensulfuronmethy[J].Pedosphere,2008,18(5):554-563. |
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| Authors: | L&#; Zhen-Mei LI Zi-Mu SANG Li-Ya and MIN Hang |
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| Institution: | College of Life Sciences, Zhejiang University, Hangzhou 310058 (China). E-mail: lzhenmei@zju.edu.cn;College of Life Sciences, Zhejiang University, Hangzhou 310058 (China). E-mail: lzhenmei@zju.edu.cn;College of Life Sciences, Zhejiang University, Hangzhou 310058 (China). E-mail: lzhenmei@zju.edu.cn;College of Life Sciences, Zhejiang University, Hangzhou 310058 (China). E-mail: lzhenmei@zju.edu.cn |
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| Abstract: | The effect of different tillage systems on the size distribution of aggregates and organic carbon distribution and storage in different size aggregates in a Hydragric Anthrosol were studied in a long-term experiment in Chongqing,China.The experiment included three tillage treatments:conventional tillage with rotation of rice and winter fallow (CT-r) system,no-till and ridge culture with rotation of rice and rape (RT-rr) system,and conventional tillage with rotation of rice and rape (CT-rr) system.The results showed that the aggregates 0.02-0.25 mm in diameter accounted for the largest portion in each soil layer under all treatments.Compared with the CT-r system,in the 0-10 cm layer,the amount of aggregates＞0.02 mm was larger under the RT-rr system,but smaller under the CT-rr system.In the 0-20 cm layer,the organic carbon content of all fractions of aggregates was the highest under the RT-rr system and lowest under the CT-rr system.The total organic carbon content showed a positive linear relationship with the amount of aggregates with diameter ranging from 0.25 to 2 ram.The storage of organic carbon in all fractions of aggregates under the RT-rr system was higher than that under the CT-r system in the 0-20 cm layer,but in the 0-60 cm soil layer,there was no distinct difference.Under the CT-rr system,the storage of organic carbon in all fractions of aggregates was lower than that under the CT-r system;most of the newly lost organic carbon was from the aggregates 0.002-0.02 and 0.02-0.25 mm in diameter. |
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| Keywords: | aggregates Hydragric Anthrosol organic carbon tillage system |
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