| 猪MYL4基因的克隆及其在猪胚胎骨骼肌中的差异表达分析 |
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| 引用本文： | 伍晓雄,唐中林,邓宏,李勇,储明星,马月辉,李奎.猪MYL4基因的克隆及其在猪胚胎骨骼肌中的差异表达分析[J].农业生物技术学报,2008,16(4). |
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| 作者姓名： | 伍晓雄 唐中林 邓宏 李勇 储明星 马月辉 李奎 |
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| 作者单位： | 华中农业大学动物遗传育种教育部重点实验室 |
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| 摘 要： | 克隆了猪MYL4基因的cDNA序列，并利用生物信息学方法进行验证。所得cDNA序列全长1105bp，包含一个完整的开放阅读框，编码197个氨基酸。序列分析表明，该基因与已报道的狗、人、黑猩猩和恒河猴等物种的MYL4基因高度同源，氨基酸序列同源性分别为98.4%、95.9%、95.4%和95.4%。该基因编码的蛋白具有EFH和FRQ1保守功能域。荧光定量分析该基因在猪胚胎骨骼肌（妊娠33 、65 和90 天）中的表达规律，发现MYL4基因在通城猪和长白猪中呈波浪式表达模式，在妊娠第33 天时中外猪品种间无显著差异，但在65 和90 天时长白猪中显著高于通城猪。
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| 关 键 词： | 猪 MYL4基因 序列分析 表达 胚胎骨骼肌 |
| 收稿时间： | 2007-10-17 |
| 修稿时间： | 2008-1-2 |
Cloning and differently expression analysis of porcine MYL4 gene in embryo skeletal muscle |
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| Abstract: | The cDNA sequences of porcine MYL4 gene were cloned, and verified by bioinformatics method. The cDNA fragment was 1105 bp with an entire open reading frame (ORF), encoding 197 amino acids. The analysis of sequence revealed that the porcine MYL4 gene was highly homologous with that of other species such as canis lupus familiaris (98.4%), Homo sapiens (95.9%),chimpanzee (95.4%) and rhesus monkey(95.4%) in amino acid. The protein encoded by porcine MYL4 gene had conserved domain ‘EFH’ and ‘FRQ1’. The expression analysis of MYL4 gene was carried out in the fetus skeletal muscle (at 33, 65 and 90 days post coitus, dpc) by real-time quantitative PCR. The results indicated that MYL4 gene was expressed with wave model in both Tongcheng and Landrace pigs. There was significant difference at 65 and 90 dpc, but no significant difference at 33 dpc between Tongcheng and Landrace. |
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