Pathogenicity and RAPD analysis of Phytophthora nicotianae pathogenic to pepper in Tunisia |
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| Authors: | Trabelsi Darine MB Allagui M Rouaissi A Boudabbous |
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| Institution: | aLaboratoire de Protection des Végétaux, Institut National de Recherche Agronomique de Tunisie, Rue Hedi Karray, 2080 Ariana, Tunisia;bLaboratoire de Microbiologie, Faculté des Sciences de Tunis, Campus Universitaire, El Manar Tunis, Tunisia |
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| Abstract: | Nine isolates of Phtophthora nicotianae were isolated from infected pepper plants. Their pathogenicity was studied in Capsicum annuum in comparison with P. nicotianae isolates from tomato and tobacco. The pathogenicity test showed that pepper isolates of P. nicotianae are adapted to their host. Banding patterns obtained by RAPD analysis with six oligonucleotide primers revealed polymorphism that grouped the isolates independently of the plant host. The polygenic dendrogram showed that pepper isolates were more similar to tomato isolates than to tobacco isolates. The RAPD bands of 1300 and 1500 bp, detected with primers OPD-01 and OPD-10, respectively, appeared specific to the most pathogenic pepper isolates. The OPK-08-1950 seems specific to the isolates of P. nicotianae from tomato. These results suggest that host specified might occur in P. nicotianae and that may be due to interspecific hybridization events resulting in novel pathogenic behavior. |
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| Keywords: | Phytophthora nicotianae RAPD pathogenicity |
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