| 凡纳滨对虾兰尼定受体基因单核苷酸多态性与对温度变化敏感性的关联分析 |
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| 引用本文： | 曾地刚,马 宁,谢达祥.凡纳滨对虾兰尼定受体基因单核苷酸多态性与对温度变化敏感性的关联分析[J].江西农业学报,2014(1):89-91. |
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| 作者姓名： | 曾地刚 马 宁 谢达祥 |
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| 作者单位： | 广西水产科学研究院广西水产遗传育种与健康养殖重点实验室,广西南宁530021 |
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| 基金项目： | 国家自然科学基金项目（31160531）;广西科技攻关项目（桂科攻11107012-1-2）资助. |
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| 摘 要： | 用直接测序法检测凡纳滨对虾兰尼定受体基因的单核苷酸多态性(SNP)位点,结果发现了1个SNP位点。通过进行凡纳滨对虾温度变化刺激的实验,把192尾对虾分为应激敏感组和应激抵抗组,用PCR产物直接测序法检测各对虾样品兰尼定受体基因的SNP的基因型。结果发现,应激敏感组和应激抵抗组的SNP等位基因频率差异显著(P<0.01)。结果显示兰尼定受体基因单核苷酸多态性与凡纳滨对虾对温度变化敏感性关联。
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| 关 键 词： | 凡纳滨对虾 兰尼定受体基因 单核苷酸多态性 应激敏感性 |
Analysis of Correlation between Single Nucleotide Polymorphism of Ryanodine Receptor Gene and Susceptibility to Temperature Change in Litopenaeus vannamei |
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| Institution: | ZENG Di - gang, MA Ning, XIE Da - xiang ＊ ( Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China) |
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| Abstract: | A single nucleotide polymorphism （SNP） locus was found in the ryanodine receptor gene of Litopenaeus vannamei by using the direct sequencing technique. A temperature change experiment was performed to divide 192 prawns into a stress - suscepti- ble group and a stress - resistant group, and the SNP genotype of ryanodine receptor gene in each prawn sample was detected by di- rect sequencing PCR product. The results showed that there was significant difference in the frequency of SNP alleles between the stress - susceptible group and stress - resistant group （ P 〈 0.01 ） , suggesting that the SNP of ryanodine receptor gene is related with the susceotibilitv of Litooenaeus vannarnei to temoerature ehange. |
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| Keywords: | Litopenaeus vannamei Ryanodine receptor gene Single nucleotide polymorphism Stress - susceptibility |
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