Antemortem Diagnosis of CDV Infection by RT-PCR in Distemper Dogs with
Neurological Deficits without the Typical Clinical Presentation |
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| Authors: | A M Amude A A Alfieri A F Alfieri |
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| Institution: | (1) Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva (DMVP), Centro de Ciências Agrárias (CCA), Universidade Estadual de Londrina, Londrina, Brazil |
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| Abstract: | In dogs with neurological disturbances without myoclonus and extraneural signs, the clinical diagnosis of distemper is difficult
perform. Considering the great infectious potential of the disease, the possibility of carrying out an antemortem diagnosis
of distemper is important, particularly in hospitalized patients with neurological disease. The present study was carried
out to evaluate RT-PCR for antemortem CDV detection in hospitalized dogs with neurological disturbances without the typical
findings of distemper. We investigated five dogs with canine distemper virus (CDV) encephalomyelitis, in which the clinical
diagnosis was not performed owing to the absence of characteristic signs of the disease, such as myoclonus and systemic signs.
We observed an apparent high sensitivity of RT-PCR in urine samples for detection of CDV: four out of five urine samples were
RT-PCR positive. The results of the present study suggest that urine is a good biological sample for antemortem CDV detection
by RT-PCR in dogs with distemper encephalomyelitis in which the clinical diagnosis is likely to be difficult owing to the
absence of suggestive distemper signs. The use of two different body fluids (urine and CSF) may increase the RT-PCR sensitivity
for antemortem diagnosis of distemper in such cases. |
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| Keywords: | dogs distemper canine distemper virus neurological signs RT-PCR |
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