| 一种适宜于文库构建的土壤微生物总DNA提取方法 |
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| 引用本文： | 高岳.一种适宜于文库构建的土壤微生物总DNA提取方法[J].安徽农业科学,2014(34):12041-12042. |
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| 作者姓名： | 高岳 |
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| 作者单位： | 苏州农业职业技术学院,江苏苏州,215008 |
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| 摘 要： | 采用改良的方法提取2种土壤微生物总DNA,并采用透析法对提取的DNA进行纯化,与MO BIO公司PowerSoil DNA isolation Kit试剂盒法进行比较,结果表明,改良的土壤微生物总DNA提取方法提取到的DNA明显高于试剂盒提取的DNA,且A260/A280及A260/A230与试剂盒提取的相差不大,片段也较大,是一种经济方便的土壤微生物总DNA提取方法,比较适合用于构建高质量的宏基因组文库.
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| 关 键 词： | 土壤微生物 透析法 DNA含量 宏基因组文库 |
A Suitable Total Microbial DNA Extraction Method for Building Genomic Library |
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| GAO Yue.A Suitable Total Microbial DNA Extraction Method for Building Genomic Library[J].Journal of Anhui Agricultural Sciences,2014(34):12041-12042. |
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| Authors: | GAO Yue |
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| Institution: | GAO Yue;Suzhou Polytechnic Institute of Agriculture; |
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| Abstract: | Improved method was used to extract soil microbial total DNA,and the dialysis method was adopted to purify DNA.Compared with PowerSoil DNA isolation Kit of MO BIO company,the obtained DNA was analyzed.DNA per gram of soil is obviously higher than that of kit method.A260/A280 and A260/A230 also achieves the ideal level,the segment is larger and less impurities,indicating that it is an economic and convenient soil microbes total DNA extraction method,and is more suitable for building high quality metagenomic library. |
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| Keywords: | Soil microbes Dialysis method DNA content Metagenomic library |
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