Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies |
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| Authors: | Vidyani?Manatunga Hossain?Sanati Peiling?Tan Email author" target="_blank">Philip?A?O’BrienEmail author |
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| Institution: | (1) School of Biological Sciences and Biotechnology, Murdoch University, Murdoch, WA, 6150, Australia;(2) National Research Center for Genetic Engineering and Biotechnology, P.O.Box 14155-6343, Tehran, Iran |
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| Abstract: | Polyclonal antiserum from mice immunized with extracellular proteins from Rhizoctonia solani inhibited pectinase and cellulase activities in cell free culture supernatants of Rhizoctonia solani. Spleen mRNA from these mice was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated using phage display techniques. Soluble ScFv antibodies produced by individual clones in Escherichia coli inhibited polygalacturonase in the culture supernatants of a range of fungal pathogens, including ascomycetes, basidiomycetes, and oomycetes. The soluble antibodies also inhibited maceration of potato tissue by these pathogens. |
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| Keywords: | biotrophs fungal pectinases maceration necrotrophs polygalacturonase recombinant antibodies |
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