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鸡伤寒白痢沙门氏菌培养基和培养方法的研究
引用本文:成进,沙依然古丽,依米提·铁木尔,谷文喜,李文蓉.鸡伤寒白痢沙门氏菌培养基和培养方法的研究[J].中国预防兽医学报,2001,23(2):137-140.
作者姓名:成进  沙依然古丽  依米提·铁木尔  谷文喜  李文蓉
作者单位:1. 新疆畜牧科学院兽医研究所,
2. 新疆畜牧科学院农业部
摘    要:本试验用5种培养基对鸡伤寒白痢沙门氏菌(Sgp)菌株C79-20和AS96-1进行了静止培养对比试验,结果表明,改良硫代硫酸钠培养基和EMM培养基为最适合Sgp的培养基,硫代硫酸钠培养基次之,普通营养琼脂和SBG琼脂最差;对C79-13菌株用改良硫代硫酸钠培养基进行了通气搅拌培养、静止厌氧培养和静止需氧培养的对比试验及pH值对细菌生长的影响试验,结果表明,通气搅拌培养方法明显优于其它两种培养方法,调整pH的试验组菌悬液的OD值和菌数明显高于不调整pH值的试验组。

关 键 词:鸡伤寒白痢沙门氏菌  培养基  培养方法
文章编号:1008-0589(2001)02-0137-04
修稿时间:2000年8月14日

Medium and Cultivation for Avian Salmonella
CHEN Jin,Saieran Guli,Yimitee Tiemur,GU Wenxi,LI Wenrong.Medium and Cultivation for Avian Salmonella[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(2):137-140.
Authors:CHEN Jin  Saieran Guli  Yimitee Tiemur  GU Wenxi  LI Wenrong
Institution:CHEN Jin 1,Saieran Guli 1,Yimitee Tiemur 1,GU Wenxi 1,LI Wenrong 2
Abstract:The comparative test of five different medium used for S.avian C79_20 and AS96_1 was investigated.The results show that the improved sodium thiosulfate medium and EEM medium were the most optimum medium,the sodium thiosulfate medium was better and the common nutritious agar and SBG agar were the worst ones.With the improved sodium thiosulfate medium,the comparative test of three different cultivation for S.avian was also investigated in this paper.The results show that the aerating and stirring cultivation was obviously better than the other methods,or motionless anaerobic cultivation and motionless oxygen_required cultivation.And the results indicate that pH value had an effect on the bacterium growth.To regulate pH value during culturing could marked increase the OD value and the number of bacterium.
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