| 32个甜菜品种指纹图谱构建与遗传多样性分析 |
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| 引用本文： | 吴则东,王茂芊,吴玉梅,王华忠,马龙彪.32个甜菜品种指纹图谱构建与遗传多样性分析[J].中国农学通报,2015,31(12):169-174. |
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| 作者姓名： | 吴则东 王茂芊 吴玉梅 王华忠 马龙彪 |
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| 作者单位： | (;1.黑龙江省普通高校甜菜遗传育种重点实验室/黑龙江大学,哈尔滨 150080；;2.黑龙江大学农作物研究院,哈尔滨 150080；;3.农业部糖料产品质量安全风险评估实验室(哈尔滨）/中国农业科学院甜菜研究所,哈尔滨 150080） |
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| 基金项目： | 基金项目：黑龙江省普通高校甜菜遗传育种重点实验室甜菜现代产业技术体系建设项目“甜菜丰产抗病种质创新及新品种选育”(CARS-210104-01)。第一作者简介：吴则东，男，1972年出生，黑龙江依兰人，副研究员，博士，主要从事甜菜遗传及分子育种的研究。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学农作物研究院，Tel：0451-86604561，E-mail：331056376@qq.com。 |
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| 摘 要： | 为了对国外引进的甜菜品种进行鉴别以及分析不同甜菜品种之间的亲缘关系，利用SSR标记构建了32份引进甜菜品种的指纹图谱并进行了遗传多样性分析。从101对SSR引物中筛选出了21对谱带清晰、易于识别的引物，这21对引物共检测出127个等位位点，其中多态性位点为107个，多态性比率为83.6%，每对SSR引物扩增出的等位位点数为2~11个，平均每对引物扩增出6.1个基因型，扩增的条带大小在90~500 bp之间。利用3对引物SB04，S6和S7的引物组合构建的指纹图谱就能够区分32个品种。聚类分析结果表明，在遗传距离0.17处，所有的供试品种被分为3类，从分子水平上说明甜菜品种之间的遗传基础比较狭窄。聚类分析结果与甜菜品种的来源具有很好的一致性，基本上是同一公司或者同一国别的品种被聚在了一起。
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| 关 键 词： | 云南 云南 烤烟 海拔 品种 适宜性 |
| 收稿时间： | 2014/10/24 0:00:00 |
| 修稿时间： | 4/3/2015 12:00:00 AM |
Construction of Fingerprinting and Analysis of Genetic Diversity for 32 Beet Varieties |
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| Wu Zedong,Wang Maoqian,Wu Yumei,Wang Huazhong and Ma Longbiao.Construction of Fingerprinting and Analysis of Genetic Diversity for 32 Beet Varieties[J].Chinese Agricultural Science Bulletin,2015,31(12):169-174. |
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| Authors: | Wu Zedong Wang Maoqian Wu Yumei Wang Huazhong and Ma Longbiao |
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| Institution: | (;1.Key Laboratory of Sugar Beet Genetic Breeding/Heilongjiang University, Harbin 150080;2.Crop Academy of Heilongjiang University, Harbin 150080;3.Laboratory of Quality & Safety Risk Assessment for Sugar Crops Products (Harbin), Ministry of Agriculture, P. R. China/Sugarbeet Research Institute of Chinese Academy of Agricultural Sciences, 150080) |
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| Abstract: | In order to identify introduced beet varieties and analyze the genetic relationship between different beet varieties, fingerprinting and genetic relationship analysis of 32 introduced beet varieties were made by SSR primers. 21 pairs of primers with distinct band and easy to identify were screened out of 101 pairs of SSR primers. From the 21 pairs of primers, a total of 127 allelic sites were detected, of which polymorphic site came to 107, with the polymorphism rate reaching 83.6%. The allelic site amplified from each pair of SSR primer amounted to 2-11. On average, each pair of primer amplified 6.1 genotypes, and the amplified stripe size ranged from 90 to 500 bp. The fingerprinting of 32 varieties was constructed by 3 pairs of primers of SB04, S6 and S7. UPGMA cluster analysis of genetic distance showed that all of the varieties were divided into three classes at the genetic distance of 0.17. It indicated that the genetic basis of introduced varieties was narrow. The cluster analysis result well accorded with the origin of the beet variety, which showed that the beet varieties which gathered together basically came from the same company or country. |
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| Keywords: | beet introduced variety SSR markers DNA fingerprinting genetic diversity |
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