| 转三价抗虫基因棉花遗传特性研究 |
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| 引用本文： | 吴 霞,李燕娥,王娇娟,上官小霞,朱 祯.转三价抗虫基因棉花遗传特性研究[J].中国生态农业学报,2006,14(1):21-23. |
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| 作者姓名： | 吴 霞 李燕娥 王娇娟 上官小霞 朱 祯 |
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| 作者单位： | 1. 山西省农业科学院棉花研究所,运城,044000
2. 中国科学院遗传与发育生物学研究所,北京,100101 |
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| 基金项目： | 国家科技攻关项目;山西省科技攻关项目;山西省自然科学基金 |
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| 摘 要： | 研究采用农杆菌介导法将外源三价抗虫基因(Bt CpTI GNA)导入常规棉花品种中获得转基因再生株,分子检测表明外源基因已在棉花体内表达并遗传给后代材料。其转化再生株后代材料经抗性检测,进行大田选育已至F8代。PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性,转化后代材料分离有的符合孟德尔规律,有的则较偏离,其所携带的基因在转基因棉花低代材料中分离规律较为含糊,高代材料则较稳定。
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| 关 键 词： | 棉花 农杆菌介导 外源三价抗虫基因 遗传特性 标记基因 |
| 收稿时间： | 2004-06-08 |
| 修稿时间： | 2004-07-29 |
Genetic characters of transgenic upland cotton cultivars carrying triple genes(Bt+ CpTI+ GNA) |
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| WU Xi,LI Yan-E,WANG Jiao-Juan,SHANGGUAN Xiao-Xia and ZHU Zhen.Genetic characters of transgenic upland cotton cultivars carrying triple genes(Bt+ CpTI+ GNA)[J].Chinese Journal of Eco-Agriculture,2006,14(1):21-23. |
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| Authors: | WU Xi LI Yan-E WANG Jiao-Juan SHANGGUAN Xiao-Xia and ZHU Zhen |
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| Institution: | 1.Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000,China;2.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101 ,China |
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| Abstract: | Foreign triple genes(Bt CpTI GNA) resistant to cotton insects have been introduced into conventional cotton cultivars by agrobacterium tumefaciens mediated transformation and the regenerated plants have been obtained.The results of molecular detection indicate that the foreign genes have been transferred into cotton plants.The progeny materials of transgenic plants are detected by insect-resistance.Up to now,the F_8 has been got after the selection in the fields.The results of PCR molecular detection,marker genes and foreign targetgene with insect-resistance are quite regular.Some of their progenies' segregations coincide with the Mendel's regularity of segregation,but some deviate,and their segregation laws in lower transgenic generation materials are vague while those in high generation materials are stabler. |
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| Keywords: | Cotton Agorbacterium tumefaciens mediated transformation Foreign triple genes resistant to cotton insects Genetic character Marker gene |
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