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| 内蒙古白刺属植物遗传多样性的RAPD分析 | |
| 引用本文： | 宛涛,蔡萍,伊卫东,张晓明,张辰波.内蒙古白刺属植物遗传多样性的RAPD分析[J].干旱地区农业研究,2007,25(1):225-229. |
| 作者姓名： | 宛涛 蔡萍 伊卫东 张晓明 张辰波 |
| 作者单位： | 1. 内蒙古农业大学生物工程学院,内蒙古,呼和浩特,010018 2. 锡林浩特牧业学院,内蒙古,锡林浩特,026000 3. 呼伦贝尔大学,内蒙古,海拉尔,021008 |
| 摘 要： | 利用随机扩增DNA多态性 (RAPD)技术对4种不同地理种群的白刺属(Nitraria L.)植物进行了遗传多样性分析.15个引物扩增出118个位点,其中多态性位点95个(80.5%),各引物的Nei's指数和Shannon's 多样性指数差别较大,利用UPGMA方法构建分子系统树.结果表明:白刺属植物的遗传分化程度和基因流因种而异,除球果白刺以外,其余3种白刺的种群间有很大的基因流,其遗传一致度在0.8561～0.9694之间,而种间遗传分化程度较低,故4种白刺有着相近的祖先,这与形态学和细胞学的分析结果一致,说明RAPD分析方法可从分子生物学角度为白刺属植物的系统学分类提供更为有利和可靠的证据. |
| 关 键 词： | 白刺属 RAPD分析 亲缘关系 遗传变异 |
| 文章编号： | 1000-7601（2007）01-0225-05 |
| 修稿时间： | 2006-04-10 |
RAPD analysis on genetic diversity of nitavia in inner mongolia |
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| WAN Tao,CAI Ping,YI Wei-dong,ZHANG Xiao-ming,ZHANG Chen-bo.RAPD analysis on genetic diversity of nitavia in inner mongolia[J].Agricultural Research in the Arid Areas,2007,25(1):225-229. | |
| Authors: | WAN Tao CAI Ping YI Wei-dong ZHANG Xiao-ming ZHANG Chen-bo |
| Institution: | 1. Inner Mongolia Agriulture University, Hohhot 010018, China ; 2. Xilinhaohe Animal Husbandry College, Xilinhaohe 026000, China ; 3. Hulunbeier University, Hailaer 021008, China |
| Abstract: | Four species of Nitraria were analyzed using random amplified polymorphic DNA(RAPD) markers.15 random primers amplified 118 loci have 95 polymorphic loci,about 80.5%.The differentees of Nei's gene diversity and Shannon's information index between each primers produced is big.Dendrogram was constructed using UPGMA.The result showed that the polarization of populations and gene fluxes are different in different species.The polarization and gene fluxes of populations of 3 Nitavias are lower except N.sphaerocarpa.The heredity coherence of each population is change from 0.8561 to 0.9694.The polarization in the populations is low,and they have the together ancestor.This is similar to morphology and cytology result,so RAPD is a useful supple mentary method in assessing genetic relationship among Nitraria species. |
| Keywords: | Nitraria L RAPD relationship genetic variation |
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