| 腹水综合征肉鸡氧自由基变化规律 |
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| 引用本文： | 吴建光,郭定宗,熊道焕.腹水综合征肉鸡氧自由基变化规律[J].中国兽医学报,2001,21(6):594-596. |
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| 作者姓名： | 吴建光 郭定宗 熊道焕 |
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| 作者单位： | 华中农业大学畜牧兽医学院,湖北,武汉,430070 |
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| 基金项目： | 湖北省科技攻关资助项目(981P0907) |
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| 摘 要： | 利用自然病例，采取分组（对照组，轻度腹水综合征组和重度腹水综合征组）对比的方法，分别用硫代巴比妥酸法和羟胺法测定了肉鸡血清和主要组织（心脏、肝脏、肺脏、肾脏）丙二醛（malondialdehyde,MDA)含量和超氧化物歧化酶（superoxide dismutase,SOD)活性。结果表明，与对照组肉鸡比较，轻度腹水综合征组肉鸡血清和主要组织MDA含量降低（P＜0．05）或显著降低（P＜0．01），SOD活性升高（P＜0．05）或显著升高（P＜0．01）；而重度腹水综合征组肉鸡血清和主要组织MDA含量升高（P＜0．05）或显著升高（P＜0．01），SOD活性显著降低（P＜0．01）。间接证明了氧自由基和氧化损伤参与了肉鸡腹水综合征的病理过程，氧自由基产生水平与肉鸡腹水综合征病情的轻重有关。
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| 关 键 词： | 肉鸡 腹水综合征 氧自由基 丙二醛 超氧化物歧化酶 |
| 文章编号： | 1005-4545(2001)06-0594-03 |
| 修稿时间： | 2000年11月12 |
Changes of Oxygen Free Radicals in Ascites Syndrome Broilers |
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| WU Jian-guang,GUO Ding-zong ,XIONG Dao-huang.Changes of Oxygen Free Radicals in Ascites Syndrome Broilers[J].Chinese Journal of Veterinary Science,2001,21(6):594-596. |
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| Authors: | WU Jian-guang GUO Ding-zong XIONG Dao-huang |
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| Institution: | WU Jian-guang,GUO Ding-zong *,XIONG Dao-huang |
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| Abstract: | The broilers were divided into three groups,including control group(C),mild ascites syndrome group(M),severe ascites syndrome group(S).Thiobarbituric acid method and hydroxylamine oxidation procedure were used respectively to determine malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in serum and major tissues(heart,liver,lung and kidney) of broilers.The results showed that MDA content decreased(P<0.05) or markedly decreased(P<0.01) while SOD activity increased(P<0.05) or obviously increased(P<0.01) in serum and major tissues of mild ascites syndrome broilers as compared with the control broilers. However,in serum and major tissues of severe ascites syndrome broilers,MDA content increased(P<0.05) or markedly increased(P<0.01) while SOD activity obviously decreased(P<0.01) as compared with the control broilers.These findings indirectly proved that oxygen free radicals and oxidation injury may play an important role in the pathological process of broiler ascites syndrome and the oxygen free radicals level is relevent to the severity of broiler ascites syndrome. |
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| Keywords: | ascites syndrome broiler malondialdehyde superoxide dismutase |
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