| 赤眼鳟形态特征及其两种同工酶的组织特异性分析 |
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| 引用本文： | 张涛,陈建武,张林,周剑光,何力.赤眼鳟形态特征及其两种同工酶的组织特异性分析[J].南方农业学报,2017,48(12):2281-2287. |
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| 作者姓名： | 张涛 陈建武 张林 周剑光 何力 |
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| 作者单位： | 农业部水产品质量安全风险评估实验室(武汉)/农业部淡水鱼类种质监督检验测试中心/中国水产科学研究院长江水产研究所,武汉,430223 |
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| 基金项目： | 国家农产品质量安全风险评估重大专项项目 |
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| 摘 要： | 目的]从形态特征和生化遗传层面上丰富赤眼鳟种质资源研究数据,同时为筛选出鉴定赤眼鳟种群的生化遗传标记打下基础.方法]采用形态学测量观察赤眼鳟的形态特征,并以聚丙烯酰胺凝胶电泳检测其不同组织(心脏、眼睛晶状体、肌肉、肝脏和肾脏)中的乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH),分析两种同工酶表达的组织特异性.结果]赤眼鳟的眼上缘可见一红斑,鳃耙短而疏,主要形态特征为背鳍D.iii-7,胸鳍P.i-14,臀鳍A.iii-7~8,腹鳍V.i-8,其齿式2·4·5/4·4·2或2·4·4/4·4·2.从赤眼鳟的心脏、眼睛晶状体、肌肉、肝脏和肾脏等5种组织中共检测到7条LDH酶带和6条MDH酶带,不同组织中的酶带数目和活性各不相同,呈明显的组织特异性,且同一组织不同个体间也存在明显差异.结论]同工酶表达的组织特异性除了受遗传基因调控外,还与各组织的生化代谢活动相关,其中心脏LDH可作为鉴定赤眼鳟种质的生化遗传标记.
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| 关 键 词： | 赤眼鳟 形态特征 乳酸脱氢酶(LDH) 苹果酸脱氢酶(MDH) 组织特异性 |
Morphological characteristics of Squaliobarbus curriculus and tissue specificity analysis for two isozymes |
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| ZHANG Tao,CHEN Jian-wu,ZHANG Lin,ZHOU Jian-guang,HE Li.Morphological characteristics of Squaliobarbus curriculus and tissue specificity analysis for two isozymes[J].Journal of Southern Agriculture,2017,48(12):2281-2287. |
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| Authors: | ZHANG Tao CHEN Jian-wu ZHANG Lin ZHOU Jian-guang HE Li |
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| Abstract: | Objective]The purpose of the present work was to enrich the research data about germplasm resources of Squaliobarbus curriculus from morphological characteristics and biochemical genetic levels,and to lay the foundation for screening out of the biochemical genetic marker for identifying the population of S.curriculus.Method]The morphologi-cal characteristics of S.curriculus were observed by morphological measurement.Lactate dehydrogenase(LDH)and ma-late dehydrogenase(MDH)isozymes from five tissues(heart,eye,muscle,liver and kidney)were detected by polyacryla-mide gel electrophoresis,and the tissue specificities expressed by two kinds of isozymes were analyzed.Result]There was a red spot on upper edge of S. curriculus eys,and their gill raker was short and sparse. The main morphological characteristics of S.curriculus included:dorsal fin D.iii-7,pectoral fin P.i-14,anal fin A.iii-7-8,and ventral fin V.i-8.The dental formula was 2·4·5/4·4·2 or 2·4·4/4·4·2.Seven LDH enzyme bands and six MDH enzyme bands were detected in heart,eye,muscle,liver and kidney of S. curriculus. The numbers and activities of the enzyme bands in different tissue were different,showing obvious tissue specificity.There were also obvious differences in the same organs between different individuals.Conclusion]The tissue specificities expressed by isozymes are not only regulated by genetic genes, but also relate to the biochemical metabolic activities of various tissues.LDH isozymes from heart can be used as bioche-mical genetic marker of S.curriculus. |
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