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| 猪圆环病毒2型陕西株的分离鉴定 | |
| 引用本文： | 杨增岐,田小艳,张 涌.猪圆环病毒2型陕西株的分离鉴定[J].西北农林科技大学学报(社会科学版),2007,35(7):7-10. |
| 作者姓名： | 杨增岐 田小艳 张 涌 |
| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西,杨凌,712100 2. 陕西省畜牧兽医总站,陕西,西安,710016 3. 榆林市榆阳区动物检疫站,陕西,榆林,719000 |
| 摘 要： | 根据GenBank中猪圆环病毒1型(PCV1)和2型(PCV2)的序列,设计2对PCR引物,对疑似PCV2感染猪的血液、肺脏和淋巴结等病料进行套式PCR扩增检测,并对PCR检测为PCV2阳性的病料进行PCV2分离与鉴定。结果显示,PCV2在PK-15细胞上生长良好,但增殖较慢且不产生细胞病变;在第12代细胞培养物中用间接免疫荧光试验(IFA)和PCR扩增反应均能检测到PCV2,且特异性很强。表明试验分离的病毒为PCV2。 |
| 关 键 词： | PCV2 PK-15 间接免疫荧光 PCR |
| 文章编号： | 1671-9387（2007）07-0007-04 |
| 收稿时间： | 9/8/2006 12:00:00 AM |
| 修稿时间： | 2006-09-08 |
Isolation and identification of PCV2 in Shaanxi |
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| YANG Zeng-qi,TIAN Xiao-yan,ZHANG Yong,ZHU Wei-guo,WANG Xu-rong,XIE Jun-liang,YANG Yong-ning,ZHANG Jin-jun.Isolation and identification of PCV2 in Shaanxi[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(7):7-10. | |
| Authors: | YANG Zeng-qi TIAN Xiao-yan ZHANG Yong ZHU Wei-guo WANG Xu-rong XIE Jun-liang YANG Yong-ning ZHANG Jin-jun |
| Institution: | 1 College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Shaanxi Animal Husbandry and Veterinary Station,Xi’an,Shaanxi 710016,China;3 Yuyang Inspection and Quarantine Bureau,Yulin,Shaanxi 719000,China) |
| Abstract: | Two pairs of primers are designed according to the sequences of PCV1 and PCV2 in the GenBank to detect PCV2 in the blood,lungs and lymph nodes of borderline cases by nest PCR. Then the PCV2 positive samples are used to separate the virus. Results show that PCV2 are well cultivated on the PK-15 cell,but there is no CPE. PCV2 can be found on the twelfth generation of the cell by IFA and PCR,and the specificity is very high,which indicates the virus separated in this experiment is PCV2. |
| Keywords: | PCV2 PK-15 PCR |
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