Ovulation rate,latency period and ova viability after GnRH- or hCG-induced breeding in the Asian catfish Pangasius hypophthalmus(Siluriformes,Pangasiidae) |
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| Institution: | 1. CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental and ICBAS, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal;2. REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal;3. ACC, A. Coelho & Castro, Lda, Praça Luís de Camões, 15 R/C, 4490-441 Póvoa de Varzim, Portugal;4. REQUIMTE/DGAOT, Faculdade de Ciências, Universidade do Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 7, 4485-661 Vila do Conde, Portugal;1. Fisheries Faculty, Department of Aquaculture, Munzur University, 62000 Tunceli, Turkey;2. Engineering Faculty, Department of Bioengineering, Munzur University, 62000 Tunceli, Turkey;3. Fisheries Faculty, Department of Basic Sciences, Munzur University, 62000 Tunceli, Turkey;1. Young Researchers and Elite Club, Lahijan Branch, Islamic Azad University, Lahijan, Iran;2. Fisheries Department, Faculty of Natural Resources, Islamic Azad University of Bandar Abbas, Bandar Abbas, Hormozgan, Iran;3. Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran;1. Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata 700094, India;2. Department of Veterinary Pharmacology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata 700037, India;3. Fish Processing Division, ICAR-Central Institute of Fisheries Technology, Willington Island, Cochin 682029, India;4. Aquatic Animal Health and Environment Division, ICAR-Central Institute of Brackishwater Aquaculture, Raja Annamalai Puram, Chennai 600028, India |
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| Abstract: | Over a 3-year period at the Sukamandi station (West Java, Indonesia), 107 Pangasius hypophthalmus females were selected on the basis of a modal oocyte diameter greater than 1.0 mm and treated with either Ovaprim (n = 97) or hCG (n = 10) to induce oocyte maturation and ovulation. The two hormonal treatments led to similar results in terms of ovulation rate (88 and 90 %), hatching rate (72 ± 25 and 82 ± 11 %) and relative fecundity (171 000 ± 73 000 and 128 000 ± 60 000 ova·kg–1, with Ovaprim and hCG, respectively). The latency period between the last hormone injection and ovulation was negatively correlated to water temperature but showed important variations at a same temperature depending on individual females (e.g. between 5 and 11 h at 28–29 °C). The ovulation time was therefore difficult to predict accurately in this species. The assessment of the viability of ova retained in the ovarian cavity after ovulation showed that the process of overripening occurs rapidly in P. hypophthalmus. The overall quality of ova began to decline as early as 2 h after ovulation and, after 3 h, hatching rates decreased and the proportion of deformed larvae increased significantly in comparison to those observed at the time of ovulation. In some individual females this process occurred even more rapidly, with a sharp decrease in hatching rates between 1 and 2 h post-ovulation. The duration of ova survival did not appear to depend on the type of hormone treatment used to induce ovulation (Ovaprim or hCG). For optimized gamete management in hatcheries, it is therefore recommended to check carefully the females for the occurrence of ovulation (between 3 and 11 h after the last hormone injection, depending on water temperature) and to strip and fertilize the eggs less than 2 h thereafter. |
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