| 两株H6N2亚型鸡源禽流感病毒的分离鉴定 |
| |
| 引用本文： | 贾宝琴,焦培荣,韦良孟,单芬,袁润余,徐成刚,廖明.两株H6N2亚型鸡源禽流感病毒的分离鉴定[J].中国动物传染病学报,2012(2):71-74. |
| |
| 作者姓名： | 贾宝琴 焦培荣 韦良孟 单芬 袁润余 徐成刚 廖明 |
| |
| 作者单位： | 华南农业大学兽医学院 农业部兽用疫苗创制重点实验室 广东省动物源性人兽共患病预防与控制重点实验室 |
| |
| 基金项目： | 国家肉鸡产业技术体系项目(nycytx-42-G3-03);广东省自然科学基金项目(10251064201000004和10151064201000021);国家自然基金项目(31172343);国际合作项目(2010DFA31000);广东省科技计划项目(20100206和2010B020307005);2010年度广东省高层次人才项目 |
| |
| 摘 要： | 本研究利用血凝抑制试验(HI)、反转录-聚合酶链式反应(RT-PCR)、基因测序等方法,对广东省某活禽交易市场进行流行病学调查时获得的两株非H5、H9亚型禽流感病毒65株和C7株进行了亚型鉴定。结果表明这两株病毒均具有血凝活性,且能被抗H6亚型禽流感病毒标准阳性血清特异性抑制。用针对禽流感病毒的M基因、H6亚型禽流感病毒HA基因、N2亚型禽流感病毒NA基因特异性鉴定引物对65株和C7株进行RT-PCR扩增,分别获得特异性目的片段。测序及BLAST分析表明两株分离株与H6N2亚型禽流感广东分离株的HA基因和NA基因核苷酸序列相似性均高达95%以上。将该两分离株鉴定为H6N2亚型禽流感病毒,并命名为A/Chicken/Guangdong/65/2009、A/Chicken/Guangdong/C7/2009。
|
| 关 键 词： | 禽流感病毒 H6N2亚型 鸡 分离鉴定 |
IDENTIFICATION OF TWO H6N2 SUBTYPE INFLUENZA VIRUSES ISOLATED FROM CHICKENS |
| |
| JIA Bao-qin,JIAO Pei-rong,WEI Liang-meng,SHAN Fen,YUAN Run-yu, XU Cheng-gang,LIAO Ming.IDENTIFICATION OF TWO H6N2 SUBTYPE INFLUENZA VIRUSES ISOLATED FROM CHICKENS[J].Chinese Journal of Animal Infectious Diseases,2012(2):71-74. |
| |
| Authors: | JIA Bao-qin JIAO Pei-rong WEI Liang-meng SHAN Fen YUAN Run-yu XU Cheng-gang LIAO Ming |
| |
| Institution: | (MOA Key Laboratory for Animal Vaccine Development,Key Lab of Zoonoses Control and Prevention of Guangdong, College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642 China) |
| |
| Abstract: | Two Avian influenza viruses 65 and C7 were isolated from chickens at a live bird market in Guangdong Province.Both strains were determined not to belong to H5 or H9 subtype.These two viruses had hemagglutination activity,which was inhibited by antiserum to Avian influenza virus H6 subtype.Then,special segments were amplified using primers specific for M gene of Influenza virus and HA and NA genes of H6N2 Avian influenza virus and blasted in GenBank of NCBI for genetic analysis.Both viruses shared more than 95% homology with H6N2 Avian influenza viruses.Therefore,the viruses 65 and C7 were classified as H6N2 subtype and designated as Chicken/Guangdong/65/2009 and A/Chicken/Guangdong/C7/2009,respectively. |
| |
| Keywords: | Avian influenza virus H6N2 chicken identification |
| 本文献已被 CNKI 等数据库收录！ |
