Molecular mapping of a new gene for resistance to rice blast (Pyricularia grisea Sacc.) |
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| Authors: | Sang-Nag Ahn Yeon-Kyu Kim Ha-Cheol Hong Seong-Sook Han Soo-Jin Kwon Hae-Chune Choi Huhn-Pal Moon Susan R McCouch |
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| Institution: | (1) Rice Breeding Division, National Crop Experiment Station, Rural Development Administration, Suweon, 441-100, Korea;(2) National Institute of Agricultural Science and Technology, Rural Development Administration, Suweon, 441-100, Korea;(3) Department of Plant Breeding & Biometry, Cornell University, Ithaca, NY, 14853, U.S.A. |
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| Abstract: | A single dominant blast resistance gene conferring resistance to a Korean rice blast isolate was identified in rice variety
`Suweon 365'. We report the chromosomal localization and molecular mapping of this blast resistance gene designated as Pi-18, which confers resistance to Korean isolate `KI-313' of the blast pathogen. To know whether there is a relationship among
genes conditioning resistance to location-specific isolates of the blast pathogen and thereby to identify linked markers to
resistance gene for isolate KI-313 collected in Korea, RFLP markers previously reported to be linked to major blast resistance
genes in different rice germplasm and other markers mapped to nearby regions were surveyed for polymorphism between a resistant
(`Suweon 365') and a susceptible (`Chucheongbyeo') parent. Linkage associations of the RFLP markers with the resistance gene
were verified using an F2 and F3 segregating population of known blast reaction. RFLP analysis showed that Pi-18 was located near the end of chromosome 11, linked to a single copy clone RZ536 at a distance of 5.4 centiMorgans (cM) and
that this gene was different from Pi-1(t). An allelism test revealed that this gene was also different from Pi-k. Currently, a combination of RAPD and microsatellite primers is being employed to find additional markers in this region.
Tightly linked DNA markers will facilitate selection for resistant genotypes in breeding programs and provide the basis for
map based cloning of this new blast resistance gene.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Blast (Pyricularia grisea Sacc ) disease resistance microsatellite RFLP (Restrictionfragment length polymorphism) rice (Oryza sativa L ) |
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