Aluminum tolerance in barley: Methods for screening and genetic analysis |
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| Authors: | Cinara Lima Echart José Fernandes Barbosa-Neto David F Garvin Suzana Cavalli-Molina |
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| Institution: | (1) Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Cx. Postal 15053, 91501–970 Porto Alegre, RS, Brazil |
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| Abstract: | Aluminum (Al) tolerance in roots of two cultivars of barley was studied using hematoxylin staining and root re-growth procedures.
This study was performed in two F2 segregating populations originated from crosses between the tolerant FM-404 and sensitive Harrington cultivars. The F2 progeny analysed with hematoxylin staining revealed a segregation ratio of 3 tolerant: 1 sensitive, showing that the Al tolerance
is controlled by a single gene with complete dominance for tolerance. The root re-growth measures do not confirm the 3:1 ratio.
This last result can be explained due to the occurrence of genes that affect root growth rate or to the difficulties found
in the evaluation of root re-growth. Barley has a complex root system, which makes it difficult to measure root re-growth
after an extended period in nutrient solution. Due to the simplicity, reliability and better precision, the hematoxylin staining
is the best procedure to determine the Al tolerance and its inheritance in barley.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | aluminum tolerance barley genetic control Hordeum vulgare ssp vulgare |
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