| 一株水产地衣芽孢杆菌的鉴定及培养基优化 |
| |
| 引用本文： | 袁科平,邹世平,李谷,何力,周群兰.一株水产地衣芽孢杆菌的鉴定及培养基优化[J].福建农林大学学报(自然科学版),2011,40(1):69-73. |
| |
| 作者姓名： | 袁科平 邹世平 李谷 何力 周群兰 |
| |
| 作者单位： | 袁科平,YUAN Ke-ping(农业部淡水生物多样性保护与利用重点开放实验室/中国水产科学研究院长江水产研究所,湖北荆州,434000;中国水产科学研究院淡水渔业研究中心,江苏,无锡,214081;热带亚热带鱼类选育与养殖重点开放实验室/中国水产科学研究院珠江水产研究所,广东,广州,510000);邹世平,李谷,何力,ZOU Shi-ping,LI Gu,HE Li(农业部淡水生物多样性保护与利用重点开放实验室/中国水产科学研究院长江水产研究所,湖北荆州,434000);周群兰,ZHOU Qun-lan(中国水产科学研究院淡水渔业研究中心,江苏,无锡,214081) |
| |
| 基金项目： | 中国水产科学研究院基本科研业务费资助项目，热带亚热带鱼类选育与养殖重点开放实验室开放课题 |
| |
| 摘 要： | 从水产养殖环境中分离、筛选得到一株菌CJ001,经菌落形态特征、生理生化特征及16S rDNA测序,鉴定其为地衣芽孢杆菌.地衣芽孢杆菌可有效降低养殖水体中的化学需氧量(COD),48 h内COD的降解率达86.8%,水温30℃时的降解率最高,达到85.7%.为优化培养基组成,选出由单因素试验确定的营养物质(红糖、玉米粉...
|
| 关 键 词： | 地衣芽孢杆菌 水产养殖 化学需氧量 培养基优化 正交试验 |
Identification of an aquatic Bacillus licheniformis CJ001 and optimization of the culture media |
| |
| YUAN Ke-ping,ZOU Shi-ping,LI Gu,HE Li,ZHOU Qun-lan.Identification of an aquatic Bacillus licheniformis CJ001 and optimization of the culture media[J].Journal of Fujian Agricultural and Forestry University,2011,40(1):69-73. |
| |
| Authors: | YUAN Ke-ping ZOU Shi-ping LI Gu HE Li ZHOU Qun-lan |
| |
| Institution: | YUAN Ke-ping1,2,3,ZOU Shi-ping1,LI Gu1,HE Li1,ZHOU Qun-lan2(1.Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture/Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000,China,2.Freshwater Fisheries Research Center,Wuxi,Jiangsu 214081,3.Key Laboratory of Tropical & Subtropical Fish Breeding & Cultivation of Chinese Academy of Fishery Sciences,Guangzhou,Guangdong 510000,China) |
| |
| Abstract: | Strain CJ001 was isolated and selected from sediment samples of breed aquatics pond in Wuhan.Based on its major morphologic,physiology-biochemistry characteristics and 16S rDNA sequence analysis,strain CJ001 was identified as Bacillus licheniformis.The strain could degrade the chemical oxygen demand(COD) in aquacultural water effectively,especially at the time of 48 h(with 86.8% degradation rate) or under the temperature of 30 ℃(with 85.7% degradation rate).According to the results of single-factor orthogonal test for culture optimization,the orthogonal experiment with 5 factors(brown sugar,corn powder,bean,carbamide and NaH2PO4) and 4 levels was then carried out.The optimal medium was as follows: brown sugar 3%,corn powder 1%,bean 3%,carbamide 0.25% and NaH2PO4 0.15%,respectively.Under the condition,11.15×109 cfu bacteria per milliliter were obtained. |
| |
| Keywords: | Bacillus licheniformis aquaculture chemical oxygen demand media optimization orthogonal experiment |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
