| 雷公藤总DNA提取方法的研究 |
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| 引用本文： | 范文洁,洪伟,李键,陈昕,林晗,吴承祯.雷公藤总DNA提取方法的研究[J].福建林学院学报,2011,31(1). |
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| 作者姓名： | 范文洁 洪伟 李键 陈昕 林晗 吴承祯 |
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| 作者单位： | 范文洁,FAN Wen-jie(宁德市国土资源局,福建,宁德,352100);洪伟,李键,陈昕,林晗,吴承祯,HONG Wei,LI Jian,CHEN Xin,LIN Han,WU Cheng-zhen(福建农林大学森林生态研究所,福建,福州,350002;福建省高校森林生态系统过程与经营重点实验室,福建,福州,350002) |
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| 基金项目： | 国家自然科学基金，教育部博士学科点专项基金 |
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| 摘 要： | 由于雷公藤叶片富含多糖及酚类物质,要获得高质量的DNA有一定难度。本研究以雷公藤植物叶片为材料,对传统提取方法进行改进、优化,比较了4种不同的提取方法,最终获得了一种以CTAB法为基础,试剂盒纯化的获得高质量DNA的方法。试验结果表明,使用该方法从雷公藤叶片中分离的DNA,纯度高、杂质少,且相对于其他方法,试验成功率明显提高,适用性强。
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| 关 键 词： | 雷公藤 DNA提取 CTAB法 试剂盒法试验 |
Research of extraction methods for genomic DNA of the plants of Tripterygium wilfordii |
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| FAN Wen-jie,HONG Wei,LI Jian,CHEN Xin,LIN Han,WU Cheng-zhen.Research of extraction methods for genomic DNA of the plants of Tripterygium wilfordii[J].Journal of Fujian College of Forestry,2011,31(1). |
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| Authors: | FAN Wen-jie HONG Wei LI Jian CHEN Xin LIN Han WU Cheng-zhen |
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| Institution: | FAN Wen-jie1,HONG Wei2,3,LI Jian2,CHEN Xin2,LIN Han2,WU Cheng-zhen2,3(1.Ningde Municipal Bureau of Land and Resources,Ningde,Fujian 352100,China,2.Institute of Forest Ecology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,3.Fujian Universities Key Laboratory of Forest Ecological System Process and Management,China) |
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| Abstract: | Because the plants of Tripterygium are rich in amylose and phenolic compounds,it′s relatively difficult to get genomic DNA of high quality.Based on the leaves material of T.wilfordii and the basis of previous studies,the traditional extraction techno-logy has been improved and optimized,and also 4 different extraction methods have been compared.Finally a technology method of getting high quality DNA was found on the basis of CTAB method and DNA kit.The experiment results showed that the DNA extracted from the leaves of Tripterygium was purer and the success rate of experiment was obviously raised and the experiment method was also more applicable. |
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| Keywords: | Tripterygium wilfordii deoxyribonucleic acid(DNA) extraction cetyltriethylammnonium bromide(CTAB) method DNA kit |
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