Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens |
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| Authors: | V J Rejish Kumar Seo Byeong Joo Mun Mi Ran Kim Chul-Joong Lee Insun Kim Hongik Park Yong-Ha |
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| Institution: | (1) Department of Applied Microbiology and Biotechnology, Yeungnam University, Kyongsan, Kyeongbuk, 712-749, South Korea;(2) Collage of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, South Korea; |
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| Abstract: | A total of 310 bacterial strains isolated from the porcine gastrointestinal tract were tested for their activity against transmissible
gastroenteritis (TGE) coronavirus and other enteric pathogens. Based on activity, the strains Probio-38 and Probio-37 were
selected as potential probiotics and identified as Lactobacillus plantarum Probio-38 and Lactobacillus salivarius Probio-37 respectively by 16S rRNA gene sequencing. Supernatants of these strains inhibited TGE coronavirus in vitro in ST
cells, without any cytopathic effect even after 72 h of incubation. Both the strains exhibited high survival in synthetic
gastric juice. The strains were resistant to 5% porcine bile and exhibited antimicrobial activity against all the 13 enteric
bacterial pathogens tested. These strains also exhibited resistance to most of the antibiotics analyzed. The inhibition of
transmissible gastroenteritis coronavirus and enteric bacterial pathogens as well as the bile tolerance, high survival in
gastric juice, and the antibiotic resistance indicate that the two isolated bacterial strains are ideal probiotic candidates
for animal application after proper in vivo experiments. |
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