| 掌叶半夏凝集素基因表达载体的构建与鉴定 |
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| 引用本文： | 徐陶,杜娟,刘月锋,王金固,谢丽霞,李尚伟.掌叶半夏凝集素基因表达载体的构建与鉴定[J].贵州农业科学,2009,37(5). |
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| 作者姓名： | 徐陶 杜娟 刘月锋 王金固 谢丽霞 李尚伟 |
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| 作者单位： | 贵州大学,贵州山地农业病虫害重点实验室,贵州,贵阳,550025 |
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| 摘 要： | 从掌叶半夏(Pinellia pedatisecta Schott)块茎中提取总RNA,反转录合成cDNA.根据GenBank中已报道的天南星科凝集素基因序列,设计带有酶切位点的表达引物,用RT-PCR方法扩增出掌叶半夏凝集素(Pinellia pedatisecta agglutinin,PPA)基因.PPA经双酶切后与表达载体pGEX-6P-3连接,构建pGEX-PPA重组表达载体.通过PCR、双酶切及测序鉴定,结果表明,重组载体pGEX-PPA构建成功,为进一步进行PPA的原核表达及其功能研究奠定了基础.
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| 关 键 词： | 掌叶半夏 凝集素 载体构建 |
Construction and Identification of Expression Vector of Pinellia pedatisecta Agglutinin (PPA) Gene |
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| XU Tao,DU Juan,LIU Yue-feng,WANG Jin-gu,XIE Li-xia,LI Shang-wei.Construction and Identification of Expression Vector of Pinellia pedatisecta Agglutinin (PPA) Gene[J].Guizhou Agricultural Sciences,2009,37(5). |
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| Authors: | XU Tao DU Juan LIU Yue-feng WANG Jin-gu XIE Li-xia LI Shang-wei |
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| Institution: | Guizhou Key Laboratory for Plant Pest Management of Mountainous Region;Guizhou University;Guiyang;Guizhou 550025;China |
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| Abstract: | Total RNA extracted from tubers of Pinellia pedatisecta was reversely transcribed into cDNA and a pair of specific primers with restriction enzyme sites were designed according to the reported sequences of agglutinin genes from Arisaema.The Pinellia pedatisecta agglutinin(PPA) gene was amplified by RT-PCR using cDNA as the template.The digested PPA was inserted into pGEX-6P-3 and the recombination vector pGEX-PPA was established.The recombination vector pGEX-PPA was identified by PCR,restriction enzyme dige... |
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| Keywords: | Pinellia pedatisecta Schott agglutinin vector construction |
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