Environmental genome shotgun sequencing of the Sargasso Sea |
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| Authors: | Venter J Craig Remington Karin Heidelberg John F Halpern Aaron L Rusch Doug Eisen Jonathan A Wu Dongying Paulsen Ian Nelson Karen E Nelson William Fouts Derrick E Levy Samuel Knap Anthony H Lomas Michael W Nealson Ken White Owen Peterson Jeremy Hoffman Jeff Parsons Rachel Baden-Tillson Holly Pfannkoch Cynthia Rogers Yu-Hui Smith Hamilton O |
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| Institution: | Institute for Biological Energy Alternatives, 1901 Research Boulevard, Rockville, MD 20850, USA. jcventer@tcag.org |
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| Abstract: | We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity. |
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