| 固定化苏云金芽孢杆菌转化生产L-鸟氨酸 |
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| 引用本文： | 易欣欣,高秀芝,陈毅明,张骁,张鹏.固定化苏云金芽孢杆菌转化生产L-鸟氨酸[J].北京农学院学报,2014,29(1):60-63. |
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| 作者姓名： | 易欣欣 高秀芝 陈毅明 张骁 张鹏 |
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| 作者单位： | 北京农学院食品科学与工程学院,农产品有害微生物及农残安全检测与控制北京市重点实验室,食品质量与安全北京实验室,北京102206;北京化工大学生命科学与技术学院,北京,100029 |
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| 基金项目： | 现代农业产业技术体系北京市叶类蔬菜创新团队建设专项资金资助（blvt-16） |
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| 摘 要： | 研究了苏云金芽孢杆菌固定化生产技术及其利用固定化细胞转化精氨酸生产L-鸟氨酸的工艺条件,结果表明:聚乙烯醇(PVA)浓度为5%、卡拉胶浓度为2.5%的凝胶固定于硼酸浓度为30g/L,KCl浓度为30g/L固定液中4h,形成的固定化细胞凝胶球可连续使用7批次。当固定化细胞浓度为100g/L、反应液温度为30℃、反应液pH值为10.0、转化时间24h条件下,转化液中L-鸟氨酸的含量为31.04g/L。
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| 关 键 词： | L-鸟氨酸 苏云金芽孢杆菌 固定化细胞 |
Production of L-ornithine by the immobilized cells of Bacillus thuringiensis |
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| YI Xinxin,GAO Xiuzhi,CHEN Yiming,ZHANG Xiao,ZHANG Peng.Production of L-ornithine by the immobilized cells of Bacillus thuringiensis[J].Journal of Beijing Agricultural College,2014,29(1):60-63. |
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| Authors: | YI Xinxin GAO Xiuzhi CHEN Yiming ZHANG Xiao ZHANG Peng |
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| Institution: | 1. Faculty of Food Science and Engineering , Beijing University of Agriculture, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue,Beijing Laboratory of Food Quality and Safety, Beijing 102206, China; 2. College of Life Science and Technology ,Beiiing University of Chemical Technology ,Beijing 100029,China) |
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| Abstract: | Bacillus thuringiensis cells were immobilized for the production of L-arginase in order to converse L-arginine into L-ornithine.The result showed PVA （5%） and carrageenan （2.5 %） were immobilized in 30 g/L boric acid and 30 g/L KC1 for 4hrs to form immobilized cell gel globes.These gel globes could be used 7 times.A maximum L-ornithine yield of 31.04 g/L,could be obtained under optimized conditions which were as follows：L-arginine 50 g/L,immobilized cell 100 g/L,the reaction temperature 30 ℃,the reaction pH 10.0 and the conversion time 24 hrs. |
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| Keywords: | L-ornithine Bacillus thuringiensis immobilized cells |
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