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| H3N2亚型犬流感病毒的分离鉴定 | |
| 引用本文： | 佘利旋,熊永忠,远立国,袁天梅,贾坤,李守军.H3N2亚型犬流感病毒的分离鉴定[J].中国预防兽医学报,2011,33(1). |
| 作者姓名： | 佘利旋 熊永忠 远立国 袁天梅 贾坤 李守军 |
| 作者单位： | 1. 华南农业大学兽医学院,广东,广州,510642 2. 中国农业科学院哈尔滨兽医研究所,维科生物技术开发公司,黑龙江,哈尔滨,150069 3. 海珠区爱宠动物诊疗所,广东,广州,510220 |
| 基金项目： | 国家自然科学基金(30972233); 广东省科技计划项目(2008A060202017、2009B060300024); 广东省自然科学基金重点项目(8251064201000008); 教育部博士点基金(20094404110017); 重要病毒跨种间感染与传播致病的分子机制研究(2011CB504700-1) |
| 摘 要： | 为了解广州地区犬流感的流行情况,本研究采集107份犬鼻咽拭子样品和58份血清样品,SPF鸡胚分离病毒并进行抗体检测。结果显示:分离获得3株H3N2亚型犬流感病毒。分离株的EID50为10-2.42/0.1 mL～10-3.5/0.1 mL;MDT为177.6 h～192 h;对乙醚、氯仿、酸和温度均敏感;对血凝素为热不稳定型;能够凝集公鸡、豚鼠、猪和牛的红细胞。 |
| 关 键 词： | 犬流感病毒 H3N2亚型 分离鉴定 生物学特性 |
Isolation and identification of H3N2 subtype canine influenza virus |
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| SHE Li-xuan,XIONG Yong-zhong,YUAN Li-guo,YUAN Tian-mei,JIA Kun,LI Shou-jun.Isolation and identification of H3N2 subtype canine influenza virus[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(1). | |
| Authors: | SHE Li-xuan XIONG Yong-zhong YUAN Li-guo YUAN Tian-mei JIA Kun LI Shou-jun |
| Institution: | SHE Li-xuan1,XIONG Yong-zhong2,YUAN Li-guo1,YUAN Tian-mei3,JIA Kun1,LI Shou-jun1*(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China,2.Harbin Weike Biotechnology Development Company,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,3.Pet Clinic of Haizhu,Guangzhou 510220,China) |
| Abstract: | To evaluate the prevalence of canine influenza virus(CIV) in Guangzhou area,samples of 107 nasopharynx swabs and 58 serum were collected from dogs for virus isolation and antibody detection.Three H3N2 subtype CIV were isolated.The 50% embryo infectious dose(EID50) of the three isolates were 10-2.42/0.1 mL to 10-3.5/0.1 mL respectively.The mean death time(MDT) ranged from 177.6 h to 192 h.All three isolates were sensitive to ether,chloroform acidum and heat,and the isolates possessed HA activity to erythrocy... |
| Keywords: | CIV H3N2 subtype isolation and identification biological characterization |
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