| 传染性法氏囊病超强病毒株VP2基因原核表达及其免疫原性检测 |
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| 引用本文： | 朱彩霞,朱瑞良,刘冠华,翁立雪,马荣德,孙寅剑.传染性法氏囊病超强病毒株VP2基因原核表达及其免疫原性检测[J].中国预防兽医学报,2011,33(2). |
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| 作者姓名： | 朱彩霞 朱瑞良 刘冠华 翁立雪 马荣德 孙寅剑 |
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| 作者单位： | 山东农业大学动物科技学院山东省动物生物技术与疫病防治重点实验室,山东,泰安,271018 |
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| 基金项目： | 高等学校博士学科点专项科研基金(060434011); 山东省科技攻关项目(2008GG10009023); 农业部公益性行业科研专项(200803019); 山东省博士后科研项目择优资助经费(200603040) |
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| 摘 要： | 为检测传染性法氏囊病超强毒株(vvIBDV)重组VP2蛋白的免疫原性,本研究利用RT-PCR方法扩增vvIBDV的结构蛋白VP2基因,并将其克隆到表达载体pGEX-4T-3中,构建重组表达质粒pGEX-VP2。将其转化受体菌E.coli BL21(DE3)plysS,经IPTG诱导后,SDS-PAGE电泳和western blot分析表明,表达的重组蛋白约69 ku,并以包涵体形式存在。表达的重组蛋白经纯化后,免疫6周龄BALB/c小鼠制备免疫血清,ELISA分析表明制备的血清效价在1∶5 120以上,表明vvIBDV VP2具有良好的免疫原性,为建立vvIBDV的ELISA检测方法提供了试验依据。
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| 关 键 词： | 传染性法氏囊病超强病毒株 VP2 E.coliBL21(DE3)plysS 表达 |
Prokaryotic expression of very virulent infectious bursal disease virus VP2 gene and immunogenic detection of expression product |
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| ZHU Cai-xia,ZHU Rui-liang,LIU Guan-hua,WENG Li-xue,MA Rong-de,SUN Yin-jian.Prokaryotic expression of very virulent infectious bursal disease virus VP2 gene and immunogenic detection of expression product[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(2). |
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| Authors: | ZHU Cai-xia ZHU Rui-liang LIU Guan-hua WENG Li-xue MA Rong-de SUN Yin-jian |
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| Institution: | ZHU Cai-xia,ZHU Rui-liang*,LIU Guan-hua,WENG Li-xue,MA Rong-de,SUN Yin-jian(Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian 271018,China) |
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| Abstract: | To express the VP2 gene of very virulent infectious bursal disease(vvIBDV),the vvIBDV VP2 gene was amplified by RT-PCR and was inserted into vector pGEX-4T-3 to construct recombinant plasmid pGEX-VP2.The pGEX-VP2 was transformed into E.coli BL21(DE3) plysS and induced with IPTG.The results of SDS-PAGE and western blot indicated that the expressed VP2 protein was about 69 ku and mainly existed in form of inclusion bodies.The anti-VP2 serum was prepared in BALB/c mice immunized with purified VP2 with the tite... |
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| Keywords: | very virulent infectious bursal disease VP2 E coli BL21(DE3) plysS expression |
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