| 玉米CCCH基因家族鉴定及分析 |
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| 引用本文： | 郭栋,宋雅菲,张佳阔,袁慧敏,杜媚,刘颖慧,袁卉馥.玉米CCCH基因家族鉴定及分析[J].中国农业科技导报,2019,21(8):19-27. |
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| 作者姓名： | 郭栋 宋雅菲 张佳阔 袁慧敏 杜媚 刘颖慧 袁卉馥 |
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| 作者单位： | 河北北方学院农林科技学院, 河北 张家口 075000 |
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| 基金项目： | 国家重点研发计划项目（2017YFD0300305）资助。 |
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| 摘 要： | CCCH锌指蛋白与植物生物和非生物胁迫、生长发育及抗病性密切相关。以玉米为材料,应用在线工具与公共数据库,共鉴定出63个CCCH基因,命名为Zm CCCHs,进一步对玉米与水稻、拟南芥、小立碗藓的CCCH基因进行生物信息学分析。结果表明:玉米的CCCH家族基因在染色体上呈不均匀分布,蛋白质氨基酸序列长度在110～962 aa之间。Zm CCCHs基因具有相对保守的结构,即包含CCCH结构域、WD40结构域、C2H2结构域、C3HC4结构域,CCCH蛋白结构中含有2个α螺旋和4个β折叠结构域。进化树分析表明玉米CCCH蛋白与水稻CCCH蛋白序列具有较高的同源性。上述结果为深入研究该基因家族的功能与表达调控提供了参考信息。
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| 关 键 词： | 玉米 CCCH基因 蛋白结构 |
Identification and Analysis of CCCH Gene Family in Maize |
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| GUO Dong,SONG Yafei,ZHANG Jiakuo,YUAN Huimin,DU Mei,LIU Yinghui,YUAN Huifu.Identification and Analysis of CCCH Gene Family in Maize[J].Journal of Agricultural Science and Technology,2019,21(8):19-27. |
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| Authors: | GUO Dong SONG Yafei ZHANG Jiakuo YUAN Huimin DU Mei LIU Yinghui YUAN Huifu |
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| Institution: | College of Agriculture and Forestry Science and Technology, Hebei North University, Hebei Zhangjiakou 07500, China |
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| Abstract: | The CCCH zinc finger protein has closely related to plant biotic and abiotic stress, growth and disease resistance. Taking corn as material, 63 CCCH genes were identified and named as ZmCCCHs by online tools and public databases. The bioinformatics analysis of CCCH genes in maize and rice, Arabidopsis thaliana and Dendrobium candidum were carried out. The results showed that the CCCHs of maize were unevenly distributed on all chromosome. The length of the protein amino acid sequences were among 110~962 aa. The ZmCCCH genes had relatively conserved structure including CCCH domain, WD40 domain, C2H2 domain and C3HC4 domain, and the CCCH proteins contained two α-helices and four β-sheet domains. Phylogenetic tree analysis indicated that maize CCCH protein had high homology with rice CCCH protein sequence. This study provided reference for further study of the function and expression regulation of this gene family. |
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| Keywords: | maize CCCH genes protein structure |
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