| GNA和ACA双价转基因烟草培育及其抗蚜研究 |
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| 引用本文： | 杨彩峰,李刚强,王楠,刘德虎.GNA和ACA双价转基因烟草培育及其抗蚜研究[J].中国农业科技导报,2019,21(4):35-41. |
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| 作者姓名： | 杨彩峰 李刚强 王楠 刘德虎 |
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| 作者单位： | 中国农业科学院生物技术研究所, 北京 100081 |
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| 基金项目： | 国家转基因生物新品种培育重大专项（2016ZX08005-004）资助。 |
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| 摘 要： | 蚜虫是一种常见的农作物害虫,作为多种植物病毒的载体而成为影响农业发展的重要害虫之一,对蚜虫的防治一直是众多科学工作者所面对的难题。构建了雪花莲凝集素(GNA)和苋菜凝集素(ACA)双价植物表达载体pBI121-GA,在农杆菌介导下,叶盘法转化烟草NC89,得到卡那霉素抗性植株65株。通过基因组PCR、ELISA、RT-PCR和Western-blot等分子生物学检测,从中筛选出外源凝集素不同表达水平的转基因烟草5株。对筛选出的5株转基因烟草植株进行初步蚜虫抗性实验,证实转基因烟草对桃蚜表现出一定的抗性,最高抑制率达85.8%,为农作物双价抗蚜基因的应用研究提供一定的理论基础。
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| 关 键 词： | 转基因烟草 雪花莲凝集素 苋菜凝集素 蚜虫抗性 |
Transgenic Tobacco Expressing GNA and ACA gene Confers the Resistance to Myzus persicae |
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| YANG Caifeng,LI Gangqiang,WANG Nan,LIU Dehu.Transgenic Tobacco Expressing GNA and ACA gene Confers the Resistance to Myzus persicae[J].Journal of Agricultural Science and Technology,2019,21(4):35-41. |
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| Authors: | YANG Caifeng LI Gangqiang WANG Nan LIU Dehu |
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| Institution: | Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Aphid is a common crop pest. As a kind of agricultural pest and the carrier of plant viruses, aphid results in serious damage in the development of agriculture and its control has always been a difficult problem faced by many scientists. A bivalent plant expression vector PB1121-GA containing the agglutinin genes of Glanthus nivalis and Amaranthus caudatus was constructed and transformed into tobacco by using Agrobacterium-mediated disc method. 65 KanR transgenic tobacco plants were regenerated. Then 5 transgenic tobacco plants expressing different levels of agglutinin were selected from these KanR plants through genomic PCR, ELISA, RT-PCR and Western-blot. A preliminary aphid bioassay experiment was carried out for investigation of the transgenic tobacco resistance to Myzus persicae, which showed that the aphid inhibition rate was significant, with the maximum reaching to 85.8%. Above results provided a theoretical basis for the research on breeding of crops resistant to aphid and the application of bivalent aphid resistance genes. |
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| Keywords: | transgenic tobacco Glanthus nivalis agglutinin Amaranthus caudatus agglutinin aphid resistance |
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