| 基因表达分析稻瘟菌7个Rho GTP酶的关系 |
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| 引用本文： | 郑武,陈继圣,郑士琴,刘文德,陈晓,周洁,鲁国东,王宗华.基因表达分析稻瘟菌7个Rho GTP酶的关系[J].中国农业科学,2006,39(11):2237-2242. |
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| 作者姓名： | 郑武 陈继圣 郑士琴 刘文德 陈晓 周洁 鲁国东 王宗华 |
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| 作者单位： | 摘要 【目的】Rho GTP酶是Ras超家族小G蛋白最主要成员。不同生物均含多种Rho GTP酶，相互之间存在密切关系，并共同参与调控多个信号转导途径。稻瘟菌基因组中存在7个Rho GTP酶，分析它们相互关系意义甚大。【方法】通过RT-PCR分析了MgCdc42不同状态下以及MgRho3插入失活状态下其它Rho GTP酶基因的表达变化。【结果】结果表明MgCdc42可能处于MgRho1、MgRho2和MgRhoX上游，对这些蛋白起正调控作用，对MgRac1和MgRho4起着负调控作用；MgRho3对MgRho1和MgCdc42的表达起正调控，对MgRac1和MgRho4的表达则起负调控作用；MgRho3与MgCdc42间关系复杂。【结论】稻瘟菌Rho GTP酶信号途径复杂，不同Rho GTP酶间存在相互调控关系，这将有助于进一步了解稻瘟菌等丝状真菌Rho GTP酶之间的相互关系。 |
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| 基金项目： | 国家自然科学基金;福建省高校科研项目 |
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| 摘 要： | 【目的】Rho GTP酶是Ras超家族小G蛋白最主要成员。不同生物均含多种Rho GTP酶，相互之间存在密切关系，并共同参与调控多个信号转导途径。稻瘟菌基因组中存在7个Rho GTP酶，分析它们相互关系意义甚大。【方法】通过RT-PCR分析了MgCdc42不同状态下以及MgRho3插入失活状态下其它Rho GTP酶基因的表达变化。【结果】结果表明MgCdc42可能处于MgRho1、MgRho2和MgRhoX上游，对这些蛋白起正调控作用，对MgRac1和MgRho4起着负调控作用；MgRho3对MgRho1和MgCdc42的表达起正调控，对MgRac1和MgRho4的表达则起负调控作用；MgRho3与MgCdc42间关系复杂。【结论】稻瘟菌Rho GTP酶信号途径复杂，不同Rho GTP酶间存在相互调控关系，这将有助于进一步了解稻瘟菌等丝状真菌Rho GTP酶之间的相互关系。
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| 关 键 词： | 稻瘟菌 RhoGTP酶 调控机制 |
| 收稿时间： | 2006-03-05 |
| 修稿时间： | 2006-03-052006-07-06 |
Relationship Among Seven Rho GTPases in Magnaporthe griesea Revealed by Gene Expression Analysis |
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| ZHENG Wu,CHEN Ji-sheng,ZHENG Shi-qin,LIU Wen-de,CHEN Xiao,ZHOU Jie,LU Guo-dong,WANG Zong-hua.Relationship Among Seven Rho GTPases in Magnaporthe griesea Revealed by Gene Expression Analysis[J].Scientia Agricultura Sinica,2006,39(11):2237-2242. |
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| Authors: | ZHENG Wu CHEN Ji-sheng ZHENG Shi-qin LIU Wen-de CHEN Xiao ZHOU Jie LU Guo-dong WANG Zong-hua |
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| Institution: | The Ministry of Education Key Laboratory of Bio-Pesticide and Chemistry Biology, Functional Genomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002 |
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| Abstract: | Objective] The Rho GTPases are major members of the Ras superfamily proteins. It is well known that there are several Rho GTPases interacting with each other and regulating several signal transduction pathways in eukaryotes. Genome analysis showed that there are seven putative Rho GTPases in Magnaporthe grisea, and it is essential to study the relationship among these Rho GTPases. Method] RT-PCR was used to analyze expression patterns of genes encoding these Rho GTPases in different MgCdc42 and MgRho3 mutants. Result] It was found that MgCdc42 positively controlled expression MgRhol, MgRho2 and MgRhoX and negatively regulated expression MgRacl and MgRho4; however MgRho3 positively regulated expressions MgRhol and MgRho2, but negatively controlled expression MgRho4 and MgRacl. The relationship between MgRho3 and MgCdc42 was complicated. Conclusion] There exists sophisticated Rho GTPase signal pathways in the fungus, and they may regulate each other between these Rho GTPases. The result will facilitate understanding the functional relationship of different GTPases in M. grisea and other filamentous fungi. |
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| Keywords: | Magnaporthe grisea Rho GTPase Gene regulation |
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