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大蒜组织培养快繁技术的研究
引用本文:胡小京,耿广东,王建龙.大蒜组织培养快繁技术的研究[J].长江蔬菜,2011(20):13-15.
作者姓名:胡小京  耿广东  王建龙
作者单位:贵州大学农学院,贵阳,550025
基金项目:科技部、财政部“富民强县”专项行动计划(205); 贵州农业科技攻关计划项目(黔科合NY字[2009]3020)
摘    要:以贵州务川紫皮大蒜茎尖为试验材料,将其接种在附加不同NAA和6-BA的MS分化培养基中,比较组织诱导、分化和生根效果。试验结果表明,以MS+6-BA 3.0 mg/L+NAA 0.5 mg/L培养基诱导愈伤组织效果最好;以MS+6-BA 2.0 mg/L+NAA 0.1 mg/L培养基诱导不定芽增殖的效果较好,增殖倍数达5倍;以MS+NAA 0.4 mg/L培养基的生根效果最佳。

关 键 词:大蒜  茎尖  组织培养  快繁

Research on Tissue Culture and Rapid Propagation Technology of Garlic
HU Xiaojing,GENG Guangdong,WANG Jianlong.Research on Tissue Culture and Rapid Propagation Technology of Garlic[J].Journal of Changjiang Vegetables,2011(20):13-15.
Authors:HU Xiaojing  GENG Guangdong  WANG Jianlong
Institution:(Agricultural College,Guizhou University,Guiyang 550025)
Abstract:The shoot tip culture of purple garlic from Wuchuan county in Guizhou province was performed on MS media supplemented with different concentration of NAA and 6-BA,to compare their effects on tissue induce,differentiation,rooting.The results showed that the most effective medium to induce callus was MS+BA 3.0 mg/L+NAA 0.5 mg/L.The most effective medium to induce adventitious buds was MS+BA 2.0 mg/L+NAA 0.1 mg/L,with the proliferation multiple of 5.The medium of MS+NAA 0.4 mg/L was the best for the root induction.
Keywords:Garlic  Shoot tip  Tissue culture  Rapid propagation
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