| 奶牛IGFBP-3基因PCR-SSCP多态性与产奶量和生长性能相关性 |
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| 引用本文： | 刘哲,吴建平,马彦男,朱静,张利平,刘晓敏,张丽.奶牛IGFBP-3基因PCR-SSCP多态性与产奶量和生长性能相关性[J].农业生物技术学报,2009,17(3):445-450. |
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| 作者姓名： | 刘哲 吴建平 马彦男 朱静 张利平 刘晓敏 张丽 |
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| 作者单位： | 甘肃农业大学 |
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| 摘 要： | 采用PCR-SSCP技术，对荷斯坦奶牛及荷斯坦公牛冻精杂交改良的黄牛后代(回交二代)共105头奶牛IGFBP-3基因第3外显子进行了多态性研究, 分析了该基因与奶牛生产性状的相关性。结果表明：IGFBP-3基因第3外显子共存在2个等位基因3种基因型，A、B基因频率及AA、AB、BB基因型的频率分别为0.7667 、0.2333和0.6000、0.3333、0.0667。该群体在这一位点上处于Hardy-Weinberg平衡状态，遗传杂合度（He）和多态信息含量(PIC)分别为0.3578和为0.2937。测序结果显示: 与AF305712相比，B等位基因在第8515碱基处发生了G-A的转换，而A等位基因在此处与AF305712序列相同；此外，A、B等位基因在AF305712的第8634碱基处均发生了T-C的无义突变。最小二乘法分析表明：AA基因型305天产奶量、12月龄体重极显著高于AB和BB基因型( P < 0.01)；AA型12月龄体长和体高均极显著高于AB型（p<0.01），而BB型与AB型和AA型差异不显著；不同基因型的胸围、腹围差异不显著。
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| 关 键 词： | 奶牛 胰岛素样生长因子结合蛋白3基因 多态性 产奶量 生长性能 |
| 收稿时间： | 2008-7-15 |
| 修稿时间： | 2008-10-4 |
PCR-SSCP Polymorphism of IGFBP-3 Gene and Its Correlation with Milk Yield and Growth Traits in Dairy Cattle |
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| Abstract: | The PCR-SSCP polymorphism of IGFBP-3 gene exon 3 and Its Correlation with milk yield and growth traits were studied in 105 Holstein cattle and its second backcross generation . The result showed: there were two alleles and three genotypes in the locus of IGFBP-3 gene exon 3. The Allelic frequencies of A and B were 0.7667 and 0.2333 , and the genotypic frequencies of AA, BB and AB were 0.6000, 0.3333 and 0.0667 respectively. The population was at Hardy-Weinberg equilibrium at this locus. The gene heterozygosity and polymorphism information content were listed for 0.3578 and 0.2937 respectively. The sequencing result showed: The B allele had happened a base transformation of G-A at the 8515th bp contrasting with the AF305712, but the A allele has same sequence in here to the AF305712; In addition, there were a no function mutation of T-C for the A and B alleles at the 8634th bp of AF305712. The study by Least Squares Method showed : the milk yield for 305 days and body weight at 12 mouth old of individuals with genotype AA was highly significantly higher than that of individuals with genotype AB and BB respectively (p<0.01). The body length and height at hip cross of individuals with genotype AA was highly significantly higher than that of individuals with genotype AB(p<0.01)，but no significant difference between BB and AB as well as between BB and AA; The genotype did not affected on heart girth and paunch girth significantly. |
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