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| 小鹅瘟病毒VP3基因的原核表达 | |
| 引用本文： | 赵文婧,鲁承,黄京爱,王暖成,朴相哲.小鹅瘟病毒VP3基因的原核表达[J].中国兽医杂志,2012,48(3):29-31. |
| 作者姓名： | 赵文婧 鲁承 黄京爱 王暖成 朴相哲 |
| 作者单位： | 1. 延边大学农学院动物医学系,吉林延吉,133000 2. 吉林省延吉市畜牧防疫站,吉林延吉,133000 3. 吉林省延吉市动物卫生检疫站,吉林延吉,133000 |
| 基金项目： | 吉林省牧业管理局项目(吉牧科字第200902) |
| 摘 要： | 根据GenBank已发表的小鹅瘟病毒B株基因序列,设计并合成1对特异性引物,经PCR扩增得到了1 457 bp的目的片段,并将该片段克隆至pMD-18T simple载体中,再将其亚克隆至原核表达载体pGEX-4T-1中,构建重组表达质粒pGEX-VP3.经0.1 mmol/L IPTG诱导,目的蛋白在大肠杆菌中得到表达.Western-blot分析表明,该蛋白具有良好的反应原性.为开发相应的基因工程疫苗奠定了理论基础. |
| 关 键 词： | 小鹅瘟病毒 VP3基因 表达 |
Expression of VP3 gene of goose parvovirus in Escherichia coli and preliminary analysis of reactogenicity |
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| ZHAO Wen-jing , LU Cheng , HUANG Jing-ai , WANG Nuan-cheng , PIAO Xiang-zhe.Expression of VP3 gene of goose parvovirus in Escherichia coli and preliminary analysis of reactogenicity[J].Chinese Journal of Veterinary Medicine,2012,48(3):29-31. | |
| Authors: | ZHAO Wen-jing LU Cheng HUANG Jing-ai WANG Nuan-cheng PIAO Xiang-zhe |
| Institution: | 1.Veterinary Medicine Department,Agricultural College of Yanbian University,Yanji 133000,China;2.Yanji Animal Husbandry Health and Quarantine Station in Jilin,Yanji 133000,China;3.Yanji Animal Health Inspection Station in Jilin,Yanji 133000,China) |
| Abstract: | According to GenBank published gosling plague virus strain B gene sequence,specific primers were designed for cloning a 1 457 bp gene fragment.The fragment was cloned to the vector pMD-18T simple,then subcloned to prokaryotic expression vector pGEX-4T-1,and constructed a prokaryotic expression plasmid pGEX-VP3.The target protein was expressed in E.coli and induced by 0.1 mmol/L IPTG.The Western-blot analysis showed that the protein has a good reactivity.This is relevant for the development of genetically engineered vaccine and has laid a solid foundation. |
| Keywords: | goose parvovirus VP3 gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |