| CHI-PAT双价基因遗传转化贵州禾来拢 |
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| 引用本文： | 李建容,曾晓芳,艾珊珊,赵德刚.CHI-PAT双价基因遗传转化贵州禾来拢[J].广西农业生物科学,2012(3):263-269. |
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| 作者姓名： | 李建容 曾晓芳 艾珊珊 赵德刚 |
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| 作者单位： | [1]贵州大学生命科学学院贵阳,550025 [2]贵州省农业生物工程重点实验室贵阳,550025 [3]教育部绿色农药与农业生物工程重点实验室,贵阳550025 |
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| 基金项目： | 本研究国家转基因新品种培育重大专项《安全转基因技术研究》子项目：转基因生物的＂基因删除＂和＂基因拆分＂技术的研究（2008ZX08010-003）资助 |
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| 摘 要： | 以贵州禾来拢幼胚为转化受体,用农杆菌介导法将几丁质酶和抗除草剂抗性双价基因（CHI-PAT）导入来拢幼胚,筛选出抗性愈伤组织并获得抗性植株.抗性植株经GUS组织化学及PCR检测呈阳性,转基因植株对50 mg/L的Basta溶液有抗性.初步证明CHI和PAT基因已整合进了水稻基因组中.
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| 关 键 词： | 贵州禾 转基因 农杆菌介导 幼胚 除草剂 |
Genetic Transformation of Rice Landrace ＂Guizhou HE Lailong＂ with CHI-PAT Bivalent Genes |
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| Li Jianrong,Zeng Xiaofang,Ai Shanshan,Zhao Degang.Genetic Transformation of Rice Landrace ＂Guizhou HE Lailong＂ with CHI-PAT Bivalent Genes[J].Journal of Guangxi Agricultural and Biological Science,2012(3):263-269. |
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| Authors: | Li Jianrong Zeng Xiaofang Ai Shanshan Zhao Degang |
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| Institution: | 1 College of Life Sciences, Guizhou University, Guiyang, 550025; 2 Guizhou Key Laboratory of Agricultural Bioengineering, Guiyang, 550025; 3 Key Laboratory of Green Pesticide and Agricultural Biological Engineering, Ministry of Education, Guiyang, 550025 |
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| Abstract: | CHI gene and PAT gene were introduced into immature embryos of Guizhou HE Lailong with Agrobacterium tumefaciens strain LBA4404. The resistant calluses were transferred into the differentiation medium and transformed plants were obtained. The expression of 13-glucuronidase （GUS） in these plants were detected and the integration of CHI was confirmed with PCR. The results of herbicide resistance test indicate that the transgenic rice with anti-herbicide gene （PA T） is resistant to 50 mg/L Basta. This research tentatively proved that CHI gene and PAT gene had been integrated into the rice genome. |
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| Keywords: | Guizhou HE Genetic transformation A grobacterium-mediated Immature embryos PPT |
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