| 拮抗BS-208菌株对番茄灰霉病诱导抗性的初步研究 |
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| 引用本文： | 杜立新,冯书亮,王容燕,王金耀,曹伟平,林开春,张用梅.拮抗BS-208菌株对番茄灰霉病诱导抗性的初步研究[J].华北农学报,2005,20(6):84-87. |
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| 作者姓名： | 杜立新 冯书亮 王容燕 王金耀 曹伟平 林开春 张用梅 |
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| 作者单位： | 河北省农林科学院植物保护研究所,河北,保定,071000;武汉天惠生物工程公司,湖北,武汉,430070 |
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| 基金项目： | 河北省农林科学院重点项目（A03-1-03-09）;武汉天惠生物工程公司合作研究项目资助 |
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| 摘 要： | 本文对拮抗BS-208菌株防治番茄灰霉病的作用机制进行了初步研究。通过在番茄苗上接种番茄灰霉病菌和拮抗枯草芽孢杆菌BS-208，测定PAL、SOD、POD和PPO的活性变化及MDA含量。结果表明，接种BS-208菌株发酵液处理48h后PAL、POD和PPO活性均升高，其中PAL和POD活性在处理后48h达到最大值，均为0．72，PPO活性在处理后96h达到最大值，为4．5，这些酶活性的升高增强了番茄抵御病原菌侵入的能力；而MDA含量与对照相比无明显变化，表明接种BS-208菌株后对番茄植株没有伤害。
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| 关 键 词： | 枯草芽孢杆菌 番茄灰霉病 诱导抗性 |
| 文章编号： | 1000-7091（2005）06-0084-04 |
| 收稿时间： | 2005-08-13 |
| 修稿时间： | 2005年8月13日 |
Preliminary Study on Induced Resistance of Antagonistic BS-208 to Tomato Grey Mould |
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| DU Li-xin,FENG Shu-liang,WANG Rong-yan,WANG Jin-yao,CAO Wei-ping,LIN Kai-chun,ZHANG Yong-mei.Preliminary Study on Induced Resistance of Antagonistic BS-208 to Tomato Grey Mould[J].Acta Agriculturae Boreali-Sinica,2005,20(6):84-87. |
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| Authors: | DU Li-xin FENG Shu-liang WANG Rong-yan WANG Jin-yao CAO Wei-ping LIN Kai-chun ZHANG Yong-mei |
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| Abstract: | Action mechanism of antagonistic BS-208 on tomato grey mould was studied.The activity changes of PAL,SOD,POD,PPO and content of MDA were tested by inoculating Botrytis cinerea and antagonistic BS-208 on tomato plants.The results showed that the activity of PAL,POD and PPO increased after inoculating strain BS-208 fermentation liquid.Among them,maximum activity of PAL and POD reached at 48 hours after the treatment were both 0.72(U),whereas,maximum activity of PPO reached at 96 hours after the treatment was 4.5(U).This increased resistance of tomato to pathogen.Moreover,the content of MDA were not significantly changed after treatment with BS-208,it indicated that tomato plants were not damaged by BS-208. |
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| Keywords: | Bacillus subtilis Tomato grey mould Induced resistance |
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