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| 适于AFLP分析用的桃成熟叶片DNA提取方法 | |
| 引用本文： | 王富荣,赵剑波,章镇,佟兆国,姜立杰,姜全,张开春.适于AFLP分析用的桃成熟叶片DNA提取方法[J].果树学报,2006,23(4):638-641. |
| 作者姓名： | 王富荣 赵剑波 章镇 佟兆国 姜立杰 姜全 张开春 |
| 作者单位： | 1. 南京农业大学园艺学院,南京,210095;北京市农林科学院林业果树研究所,北京,100093 2. 北京市农林科学院林业果树研究所,北京,100093 3. 南京农业大学园艺学院,南京,210095 |
| 摘 要： | 以多个野生桃秋季成熟叶片为材料,采用改进CTAB法对其基因组总DNA的提取进行了研究,并与其相应幼叶DNA的提取效果进行了比较分析。结果表明,从野生桃成熟叶片提取的DNA除产量低于幼叶外,DNA的纯度和完整性都较好,D260nm/D280nm的比值均为1.8～2.0,无降解现象,RNA去除干净,能被限制性内切酶完全消化;其AFLP分析(引物EcoRI-TA/MseI-CTT)条带清晰,多态性好,说明此方法提取的野生桃成熟叶片基因组总DNA完全适于AFLP分析。 |
| 关 键 词： | 桃 DNA提取 成熟叶片 AFLP |
| 文章编号： | 1009-9980（2006）04-638-04 |
| 收稿时间： | 2006-02-14 |
| 修稿时间： | 2006-05-08 |
DNA extraction method for AFLP analysis in mature leaves of peach |
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| WANG Fu-rong,ZHAO Jian-bo,ZHANG Zhen,TONG Zhao-guo,JIANG Li-jie,JIANG Quan,ZHANG Kai-chun.DNA extraction method for AFLP analysis in mature leaves of peach[J].Journal of Fruit Science,2006,23(4):638-641. | |
| Authors: | WANG Fu-rong ZHAO Jian-bo ZHANG Zhen TONG Zhao-guo JIANG Li-jie JIANG Quan ZHANG Kai-chun |
| Institution: | 1College of Horticulture ,Nanjing Agricultural University,Nanjing,Jiangsu 210095 China;2Institute of Forestry and Pomology,Beijing A- cademy of Agricultural and Forestry Sciences ,Beijing 100093 China |
| Abstract: | The genomic DNA samples of mature leaves of several wild peach varieties in autumn were isolated by improved CTAB method,and DNA extraction effects were contrasted with their tender leaves. The results showed: the extracted DNA of mature leaves by this method was pure,integral,the value of D260 nm/D280 nm was 1.80 to 2.0,degradation was nearly free,the RNA was eliminated completely,and suitable for digestion by restriction endonucleases,except the yields of DNA extraction from mature leaves was lower than that of the tender leaves. AFLP analysis indicated the primer (EcoRI-TA/MseI-CTT) produced clear polymorphic patterns. Therefore this method could be used for extracting ideal DNA samples from mature leaves of wild peach and suitable for AFLP. |
| Keywords: | Peach DNA extraction Mature leaves AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |