| 不同生长势梨种质矮化预选指标的比较研究（摘要） |
| |
| 引用本文： | 徐颖,曹后男,宗成文,巩艳明,朴永虎.不同生长势梨种质矮化预选指标的比较研究（摘要）[J].农业科学与技术,2009,10(6):108-110,143. |
| |
| 作者姓名： | 徐颖 曹后男 宗成文 巩艳明 朴永虎 |
| |
| 作者单位： | 延边大学农学院,吉林龙井,133400 |
| |
| 基金项目： | 国家自然科学基金(30560091 |
| |
| 摘 要： | 目的]为梨育种选择、选配亲本及性状的早期鉴定提供理论依据。方法]以S2×山梨（乔化）、S2×山梨（矮化）、S2、山梨、极矮化种质为材料,从叶片气孔密度、枝皮率、叶片厚度、栅栏组织厚度、栅海比、导管密度等指标来判断各种质的矮化性状。气孔密度的测定：选择实生苗主干中部、向阳面成熟叶片,10：00～10：30采叶。观察气孔时用指甲油涂抹叶背主脉中间两侧,干燥几分钟后,用镊子将指甲油层揭下,放在载玻片上,盖上盖玻片,利用Olympus显微图像分析仪分析,选择气孔密度中等的视野,观察气孔密度和大小。枝皮率的测定：选取一年生枝条10个,利用游标卡尺按十字交叉法分别测量除皮前后的枝条直径,以（1-ab/AB）×100%计算枝皮率（A、B为枝纵横径,a、b为去皮后枝纵横径）,取平均值。叶片厚度、栅栏组织厚度、栅海比及导管密度的研究：采用石蜡切片法测定。材料用FAA液固定,利用切片机切片,在DMB5、Moticam 1300显微影像系统摄影,Motic Image Advanced 3.2捕捉图像并进行显微测量。结果]5种梨种质中生长势强的山梨枝皮率（27.4%）、叶片厚度（200.5μm）、栅栏组织厚度（93.8μm）及栅海比（0.75）最小,而气孔密度（396.00个/mm~2）和导管密度（377.7个/μm~2）最大。生长势弱的梨极矮化种质枝皮率（45.0%）、叶片厚度（248.3μm）、栅栏组织厚度（140.4μm）、栅海比（1.59）最大,而气孔密度（235.75个/mm~2）和导管密度（287.0个/μm）最小。各种质在气孔密度、枝皮率、叶片厚度、栅栏组织厚度、栅海比、导管密度之间存在显著差异（P〈0.05）。结论]气孔密度、枝皮率、叶片厚度、栅栏组织厚度、栅海比以及导管密度可作为梨树体生长势早期鉴定的指标。
|
| 关 键 词： | 梨 矮化 预选指标 |
Comparative Study on the Dwarfing Pre-selected Indices of Pear Germplasms with Different Growth Potentials |
| |
| XU Ying,CAO Hou-nan,ZONG Cheng-wen,GONG Yan-ming,PIAO Yong-hu.Comparative Study on the Dwarfing Pre-selected Indices of Pear Germplasms with Different Growth Potentials[J].Agricultural Science ＆ Technology,2009,10(6):108-110,143. |
| |
| Authors: | XU Ying CAO Hou-nan ZONG Cheng-wen GONG Yan-ming PIAO Yong-hu |
| |
| Institution: | (Agricultural School of Yanbian University, Longjing 133400) |
| |
| Abstract: | Objective] The aim of this study was to provide a theoretical basis for breeding selection, matching parents and the identification of traits during early period. Method ] With Shanli （ Pyrus ussuriensis Maxim） , S2 × Shanli （vigorous）, S2 x ShanU （dwarfing）, S2, super-dwarfing germplasm as the matedais, the dwarfing traits of each germplasm were identified by indices including leaf stomata density, branch-cortex ratio, leaf thickness, palisade tissue thickness, paisade-spongy ratio and vessel density. Result] Among five kinds of pear germplasms, Shanli with strong growth potential had the smallest branch-cortex ratio, leaf thickness, palisade tissue thickness and palisade-spengy ratio, but the largest stomata density and vessel density. On the contrary, super-dwarfing germplasm with weak growth potential had the largest branch-cortex ratio, leaf thickness, palisade tissue thickness and palisade-spongy ratio, but the smallest stomata density and vessel density. There was a difference in stomata density, branch-cortex ratio, leaf thickness, palisade tissue thickness, palisade-spongy ratio and vessel density for every germplasm. Conclusion] Stomata density, branch-cortex ratio, leaf thickness, palisade tissue thickness, palisade-spongy ratio and vessel density can be used as indices of identification for pear growth potential in early period. |
| |
| Keywords: | Pear Dwarfing Pre-selected indices |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
