| 随机扩增多态性DNA技术对绿色气球菌的相关性分析 |
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| 引用本文： | 王羽,高云航.随机扩增多态性DNA技术对绿色气球菌的相关性分析[J].中国兽药杂志,2017,51(12):19-23. |
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| 作者姓名： | 王羽 高云航 |
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| 作者单位： | 吉林农业大学动物科学技术学院,吉林农业大学 |
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| 摘 要： | 为考察恩诺沙星与乙酰甲喹的联合抗菌活性,采用肉汤稀释棋盘法测定了恩诺沙星与乙酰甲喹单用及联用对大肠杆菌、沙门氏菌临床菌株的体外抗菌活性。结果显示:恩诺沙星与乙酰甲喹单独使用对大肠杆菌的最小抑菌浓度为8μg/m L和32μg/m L,对沙门氏菌的最小抑菌浓度为16μg/m L和256μg/m L。两药联用对大肠杆菌和沙门氏菌的FIC指数为0.5和0.75,分别表现为协同作用和相加作用。试验表明,恩诺沙星与乙酰甲喹联用可以增强对大肠杆菌和沙门氏菌感染的治疗效果。
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| 关 键 词： | 乙酰甲喹 恩诺沙星 联合抗菌 FIC指数 |
| 收稿时间： | 2017/3/30 0:00:00 |
| 修稿时间： | 2017/10/30 0:00:00 |
Drug Resistance Analysis of Aerococcus viridians by Random Amplified Polymorphic DNA |
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| Institution: | College of Animal Science and Technology,Jilin Agricultural University, |
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| Abstract: | With the development of molecular biology, the genes of 16S,23S and 16S-23S rRNA
have been used in strain identification.The gene intergenic spacer(ISR) of16S-23S rRNA has a
higher variability than the former two,which remedies the defects of the strong conservative and
the lack of differentiations of 16Sand 23S rRNA. In this study,we amplified the ISR of 7
Aerococus viridian strains and 2 Enterococcus faecium strains. We analysed the DNA of
Aerococus viridian strains and evaluated the clone correlations and resistances of different strains by randomly amplified polymorphic DNA(RAPD). |
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| Keywords: | Aerococcus viridians 16S-23S rRNA RAPD |
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