| 香蕉14-3-3蛋白基因Ma-14-3-3d的克隆及序列分析 |
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| 引用本文： | 李美英,徐碧玉,杨小亮,刘菊华,张建斌,金志强.香蕉14-3-3蛋白基因Ma-14-3-3d的克隆及序列分析[J].安徽农业科学,2008,36(27). |
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| 作者姓名： | 李美英 徐碧玉 杨小亮 刘菊华 张建斌 金志强 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海南海口,571101;海南大学农学院,海南儋州,571737;中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家自然科学基金，公益性行业(农业)科研专项 |
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| 摘 要： | 目的]克隆分析香蕉中14-3-3蛋白编码基因。方法]采用PCR与RACE技术相结合的方法克隆香蕉14-3-3基因,并进行CDNA测序及同源性分析。结果]所克隆cDNA全长866 bp,编码197个氨基酸残基,具有植物14-3-3蛋白基因的特征结构域,并与其他植物来源的14-3-3蛋白具有很高的序列相似性,将其命名为Ma-14-3-3d(Musa acuminate14-3-3gene)。结论]Ma-14-3-3d蛋白与来源于单子叶植物的14-3-3蛋白位于同一进化枝上。
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| 关 键 词： | 香蕉 Ma-14-3-3d 14-3-3蛋白 克隆 |
Cloning and Sequence Analysis of Ma-14-3-3d Encoding a Homologue 14-3-3 Protein from Banana |
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| LI Mei-ying et al.Cloning and Sequence Analysis of Ma-14-3-3d Encoding a Homologue 14-3-3 Protein from Banana[J].Journal of Anhui Agricultural Sciences,2008,36(27). |
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| Authors: | LI Mei-ying |
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| Abstract: | Objective] The aim of the study is to clone and analyze the gene encoding 14-3-3 protein from banana.Method] Together with PCR amplification,RACE (rapid amplification of cDNA ends) technique was employed to clone 14-3-3 gene from banana;then the amplified sequence was sequenced and homologically analyzed.Result] A new cDNA homologous with 14-3-3 protein genes were obtained by RT-PCR and RACE(rapid amplification of cDNA ends) approaches.The full length of this cDNA was 866 bp encoding 197 amino acids.Alignment of deduced amino acid sequence with those from other plants revealed that the cDNA shared high homology with 14-3-3 protein genes from other plants,and was designated as Musa acuminata 14-3-3 gene(Ma-14-3-3d).Phylogenetic analysis reveals that Ma-14-3-3d has closer genetic relationship with those from monocotyledon species than those from other species.Conclusion] Ma-14-3-3d belongs to the same lineage of 14-3-3 from monocotyledon. |
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| Keywords: | Ma-14-3-3d |
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