| 大豆脂肪氧化酶缺失体鉴定方法研究 |
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| 引用本文： | 杨柳,李文滨,师臣,田中艳,周长军,李建英,吴耀坤.大豆脂肪氧化酶缺失体鉴定方法研究[J].黑龙江农业科学,2009(4):12-14. |
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| 作者姓名： | 杨柳 李文滨 师臣 田中艳 周长军 李建英 吴耀坤 |
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| 作者单位： | 1. 黑龙江省农业科学院大庆分院,黑龙江大庆,163316
2. 东北农业大学大豆研究所/大豆生物学教育部重点实验室,黑龙江哈尔滨,150030 |
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| 摘 要： | 应用比色法和等电聚焦聚丙烯酰胺凝胶电泳法鉴定大豆脂肪氧化酶缺失体,得出结论：后者是一种简单明了的鉴定脂肪氧化酶基因缺失材料的方法,能够适应大豆育种工作要求（要使农艺性状和缺失脂肪氧化酶综合到某一个体中需要对成千上万的群体进行鉴定）;并同时筛选出了农艺性状优良,缺失脂肪氧化酶Lox2的稳定新品系F4-5。
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| 关 键 词： | 脂肪氧化酶 比色法 薄层等电聚焦聚丙烯酰胺凝胶电泳法 |
Research of Lipoxyenase Nulls Tested Method in Soybean |
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| YANG Liu,LI Wen-bin,SHI Chen,TIAN Zhong-yan,ZHOU Chang-jun,LI Jian-ying,WU Yao-kun.Research of Lipoxyenase Nulls Tested Method in Soybean[J].Heilongjiang Agricultural Science,2009(4):12-14. |
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| Authors: | YANG Liu LI Wen-bin SHI Chen TIAN Zhong-yan ZHOU Chang-jun LI Jian-ying WU Yao-kun |
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| Institution: | 1.Daqing Sub-academy of Heilongjiang Academy of Agricultural Sciences;Daqing;Heilongjiang 163316;2.Soybean Research Institute of Northeast Agricultural University;Key Laboratory of Soybean Biology of Chinese Education Ministry;Harbin;Heilongjiang 150030 |
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| Abstract: | Lipoxyenase nulls were tested by colorimetric assays and IEF-PAGE in the study.The IEF-PAGE was a simple and effective method.It could complete soybean breeding objection that must test thousands of populations.A stable line F4-5 combining lipoxygenase triple nulls with better agronomic and the quality traits had been obtained. |
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| Keywords: | lipoxyenase nulls colorimetric assays IEF-PAGE |
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