| 黄瓜栽培种、近缘野生种、种间杂种及其回交后代的RAPD 分析 |
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| 引用本文： | 庄飞云,陈劲枫.黄瓜栽培种、近缘野生种、种间杂种及其回交后代的RAPD 分析[J].园艺学报,2003,30(1):47-50. |
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| 作者姓名： | 庄飞云 陈劲枫 |
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| 作者单位： | (作物遗传与种质创新国家重点实验室, 南京农业大学蔬菜研究所, 南京210095) |
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| 基金项目： | 国家自然科学基金项目 ( 3 0 170 64 4)，国家高技术研究发展计划项目 ( 2 0 0 1AA2 4112 3 ,2 0 0 2AA2 412 5 1,2 0 0 2AA2 0 70 12 )，教育部跨世纪优秀人才培养计划项目，教育部科学技术研究重点项目 (重点 0 10 97)，教育部留学回国人员科研启动基金项目 |
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| 摘 要： | 黄瓜栽培种、黄瓜野生变种、近缘野生种、种间杂交种及其与黄瓜回交自交的后代以及甜瓜为试材, 采用RAPD 方法探讨其亲缘关系。对23 份试材进行扩增, 筛选出31 条随机引物的扩增产物进行结果分析, 共产生375 个位点, 具多态性位点占90. 0 % , 平均每条扩增出6. 3 条。通过UPGMA 聚类分析,以遗传距离0. 37 为阀值, 可将23 份试材归为黄瓜、近缘野生种、种间杂交种及甜瓜亚属种4 类。
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| 关 键 词： | 黄瓜 种间杂交 亲缘关系 RAPD |
| 文章编号： | 0513-353X(2003)01-0047-04 |
| 收稿时间： | 2002-1-23 |
| 修稿时间： | 2002年1月23日 |
RAPD Analysis of Cultivated Cucumber,Wild Cucumis Species,Interspecific Hybrid and Its Progenies from Backcrossing |
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| Zhuang Feiyun and Chen Jinfeng.RAPD Analysis of Cultivated Cucumber,Wild Cucumis Species,Interspecific Hybrid and Its Progenies from Backcrossing[J].Acta Horticulturae Sinica,2003,30(1):47-50. |
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| Authors: | Zhuang Feiyun and Chen Jinfeng |
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| Institution: | 1. Burns Department of Zhujiang Hospital, Guangzhou 510282,China;
2. Department of Pathophysiology, The First Military Medical University, Guangzhou 510515, China |
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| Abstract: | Cultivated cucumber and its wild varieties , wild Cucumis species ( C. hystrix Chakr. ) ,interspecific hybrids and the BC1S2 , and melon cultivars were used to study the relationship by RAPD markers. 31 arbitrary primers were screened to detected polymorphism among the total 23 cultigens used. Of the 375 sitesproduced , 90. 0 % were polymorphic. Each primer amplified an average of 6. 3 fragments. The results from UPGMA cluster analysis suggested that 23 cultigens could be classified into four groups , they are : cultivated cucumber and its wild variety , C. hystrix , C. ×hytivus , and melons. |
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| Keywords: | Cucumis Interspecific hybridization Phylogeny RAPD |
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