| 螯虾次目功能基因密码子偏好性研究 |
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| 引用本文： | 李喜莲,杨元杰,李倩,刘金殿,顾志敏.螯虾次目功能基因密码子偏好性研究[J].浙江农业学报,2014,26(4):862. |
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| 作者姓名： | 李喜莲 杨元杰 李倩 刘金殿 顾志敏 |
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| 作者单位： | 浙江省淡水水产研究所，浙江 湖州 313001 |
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| 基金项目： | 公益性行业(农业)科研专项经费项目(201003070);湖州市自然科学基金项目(2012YZ09) |
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| 摘 要： | 以螯虾次目功能基因组中的208个蛋白质编码基因序列为数据来源，应用CodonW 14软件对螯虾次目功能基因中密码子组成、同义密码子使用频率、密码子3个位置的G+C含量、有效密码子数等进行分析。结果显示，螯虾次目密码子第三位的G+C含量明显高于第一/二位，表现出对以G或C碱基结尾的密码子的偏好性使用，并确定了9个螯虾次目最优密码子。上述研究结果为今后螯虾次目新基因的发现、功能基因表达调控研究、蛋白质结构和功能预测、以及与其他虾类的比较基因组学研究、螯虾分子标记育种等提供了理论基础。
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| 关 键 词： | 螯虾次目 密码子偏好性 最优密码子 |
Analysis on codon bias of functional gene of Astacidea |
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| LI Xi lian,YANG Yuan jie,LI Qian,LIU Jin dian,GU Zhi min.Analysis on codon bias of functional gene of Astacidea[J].Acta Agriculturae Zhejiangensis,2014,26(4):862. |
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| Authors: | LI Xi lian YANG Yuan jie LI Qian LIU Jin dian GU Zhi min |
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| Institution: | Zhejiang Institute of Freshwater Fisheries， Huzhou 313001， China |
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| Abstract: | Coding DNA sequences (CDS) of 208 proteins in Astacidea were analyzed in the present study. The content of G+C at three positions of codons， ENC (effective number of codon) of genes and frequency of synonym codon usage were calculated by the software CodonW 14， and the “optimal codons” were also determined. The results showed that the content of G+C at the third position of codons was significantly higher than that of the first and second positions， and genes preferred to the codons with C or G at the synonymous position. In addition， 9 codons were determined as the “optimal codons”， which were ended with G or C. The data would offer references in the research on new gene search， gene expression， forecast of protein function and structure， and comparative genome with other shrimps. |
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| Keywords: | Astacidea codon bias optimal codon |
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