| 两种模式酵母中可分解丙酮醛的GLO3的特性及鉴定 |
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| 引用本文： | 彭静静.两种模式酵母中可分解丙酮醛的GLO3的特性及鉴定[J].浙江农业学报,2014,26(4):1084. |
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| 作者姓名： | 彭静静 |
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| 作者单位： | 泰山学院 生物与酿酒工程学院，山东 泰安 271021 |
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| 基金项目： | 泰安市科技发展计划(20132094);泰山学院博士科研启动金(Y-01-2013001) |
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| 摘 要： | GLO3是一种不依赖辅助因子就可分解丙酮醛的酶，有研究表明大肠杆菌的EcHsp31和人的HsDJ 1均属于这种GLO3。丙酮醛是细胞糖酵解过程中产生的一种有毒的副产物，乙二醛酶Ⅰ(GLO1)和乙二醛酶Ⅱ(GLO2)在谷胱甘肽(GSH)的辅助下可以降解细胞内丙酮醛，乙二醛酶Ⅲ(GLO3)不需要GSH的辅助可以直接降解MG。研究真菌的GLO3降解丙酮醛的机制，对认识和研究因丙酮醛引起的疾病具有重要的指导意义。研究表明，真菌中GLO3同源蛋白十分复杂，Hsp31在真菌中广泛存在，DJ 1只在部分真菌中存在，进化树结果支持GLO3的同源蛋白Hsp31和DJ 1是一种趋同进化。对模式真菌酿酒酵母的ScHsp31和裂殖酵母SpDJ 1进行GLO3的酶活测定和HPLC产物检测表明，这两种蛋白均具有GLO3的特性。
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| 关 键 词： | 真菌 丙酮醛 GLO3 趋同进化 HPLC |
Identification and characterization of glyoxalase III enzymes in two model yeast#br# |
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| PENG Jing jing.Identification and characterization of glyoxalase III enzymes in two model yeast#br#[J].Acta Agriculturae Zhejiangensis,2014,26(4):1084. |
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| Authors: | PENG Jing jing |
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| Institution: | College of Biology and Brewing Engineering， Taishan University， Taian 271021， China |
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| Abstract: | Most recently， a GSH independent glyoxalase pathway was identified in Escherichia coli， Caenorhabditis elegans， mice and humans. Mechanisms of MG detoxicification include the glutathione (GSH) dependent pathway consisting of glyoxalase Ⅰ (GLO1) and glyoxalase Ⅱ (GLO2)， and GSH independent pathway catalyzed by glyoxalase Ⅲ (GLO3). E. coli Hsp31 and metazoan DJ 1 proteins， belong to two different subfamilies of the Hsp31/DJ 1 superfamily， have been demonstrated to have GLO3 activity. However， genes encoding GLO3 have not been reported in fungi. We performed a systematic survey of homologs of Hsp31 and DJ 1 in fungi. We found that Hsp31 proteins are widely distributed among different fungal groups， whereas DJ 1 proteins have a very limited distribution in fungi. Phylogenetic analysis of these sequences revealed that fungal Hsp31 and DJ 1 proteins form two distinct monophyletic groups， and that fungal Hsp31 proteins can clearly be divided into different classes. One Saccharomyces cerevisiae Hsp31 protein (ScHsp31) and Schizosaccharomyces pombe DJ 1 (SpDJ 1) have GLO3 activity in vitro. Our results revealed that fungal homologs of the Hsp31/DJ 1 superfamily could be GLO3， which may have some roles in protecting cells from MG toxicity. Our results also support the view that GLO3 activity likely evolved through convergent evolution. |
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| Keywords: | fungi methylglyoxal GLO3 convergent evolution HPLC |
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