| 苜蓿转LEA_3基因研究 |
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| 引用本文： | 肖荷霞,朱宝成,毛彩云.苜蓿转LEA_3基因研究[J].河北农业科学,2010,14(10):73-75,83. |
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| 作者姓名： | 肖荷霞 朱宝成 毛彩云 |
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| 作者单位： | [1]沧州市农林科学院,河北沧州061001 [2]河北农业大学生命科学学院,河北保定071002 |
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| 摘 要： | 以苜蓿(Medicago sativa)品种中苜一号为试材,进行愈伤组织的诱导。通过基因枪法将来源于大麦的胚胎发生丰富蛋白基因lea3导入愈伤组织细胞,于8 mg/L PPT的选择压力下筛选2个月,获得了抗性愈伤组织及再生植株,并将再生植株再次转入含有PPT的诱导继代培养基中进行筛选。通过PCR检测,在21株再生植株中有2株扩增出了目的基因片段。证实lea3基因已导入了苜蓿细胞中,并表达。
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| 关 键 词： | 苜蓿 胚性愈伤组织 lea3基因 转化 再生 |
Study on Transgenic LEA3 Gene in Medicago sativa |
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| XIAO He-xia,ZHU Bao-cheng,MAO Cai-yun.Study on Transgenic LEA3 Gene in Medicago sativa[J].Journal of Hebei Agricultural Sciences,2010,14(10):73-75,83. |
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| Authors: | XIAO He-xia ZHU Bao-cheng MAO Cai-yun |
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| Institution: | 1.Cangzhou Academy of Agriculture and Forestry Sciences,Cangzhou 061001,China;2.College of Life Science,Hebei Agriculture University,Baoding 071002,China) |
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| Abstract: | The somatic embryo calli of Medicago sativa cv.Zhongmu No.1 were induced.LEA3 gene from barley was introduced into the calli cells by microprojectile bombardment,then it was transferred to an identical medium with 8 mg/L PPT.After screening for two months,resistant callus and regenerated plants were obtained.The regenerated plants were transferred to the regeneration medium with PPT.The results of PCR showed that 2 lines had attained Lea3 gene,and the transformation rate was nearly 10%. |
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| Keywords: | Alfalfa Embrygenic calli Lea3 gene Transformation Regeneration |
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