| 致绵羊脑炎型肠球菌溶血素CylA基因的克隆及序列测定 |
| |
| 引用本文： | 韩素娟,剡根强,周霞,王静梅,马贵军.致绵羊脑炎型肠球菌溶血素CylA基因的克隆及序列测定[J].中国预防兽医学报,2007,29(4):281-284. |
| |
| 作者姓名： | 韩素娟 剡根强 周霞 王静梅 马贵军 |
| |
| 作者单位： | 新疆石河子大学,动物科技学院/新疆地方病与民族高发病省部共建实验室,新疆,石河子,832003 |
| |
| 摘 要： | 以致绵羊脑炎型肠球菌（Enterococcus）染色体DNA为模板，PCR扩增溶血素CylA的结构基因，将PCR产物克隆于pMD19-T载体，对PCR产物进行测序鉴定。与已发表的肠球茵溶血素CylA基因序列比较，同源性达99．3％。
|
| 关 键 词： | 绵羊脑炎型肠球菌溶菌素 CylA基因克隆 测序 |
| 文章编号： | 1008-0589（2007）04-0281-04 |
| 收稿时间： | 2006-06-20 |
| 修稿时间： | 2006年6月20日 |
Cloning and analysis of DNA sequence of gene CylA of enterococci inducing sheeps Encephalitis |
| |
| HAN Su-juan,YAN Gen-qiang,ZHOU Xia,WANG Jing-mei,MA Gui-jun.Cloning and analysis of DNA sequence of gene CylA of enterococci inducing sheeps Encephalitis[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(4):281-284. |
| |
| Authors: | HAN Su-juan YAN Gen-qiang ZHOU Xia WANG Jing-mei MA Gui-jun |
| |
| Abstract: | DNA fragment of CylA gene was amplified from the chromosomal DNA of Eerococci Inducing Sheeps Encephalitis by PCR and cloned into pMDI9-T Vector and sequenced. DNA sequence comparison of the amplified fragment with the published sequence of gene CylA showed 99 % nucleotide homology. |
| |
| Keywords: | eerococci inducing sheeps encephalitis CylA cloning sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
