| 水稻白叶枯病菌及其毒素引起烟草叶片组织坏死机制的研究 |
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| 引用本文： | 宋从凤,潘小玫,杨悦.水稻白叶枯病菌及其毒素引起烟草叶片组织坏死机制的研究[J].植物病理学报,1999,29(1):58-63. |
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| 作者姓名： | 宋从凤 潘小玫 杨悦 |
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| 作者单位： | 南京农业大学植物保护系病虫监测与治理农业部重点开放实验室, 南京 210095 |
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| 摘 要： | 水稻白叶枯病菌JXO Ⅲ细胞悬浮液及其分泌的毒素溶液接种到烟草叶片中,都能在烟草叶片上产生快速的坏死反应。引起坏死反应所需的最短时间分别为接种后8 h和0.5 h。低浓度的JXOⅢ(< 107 cfu/ml)接种后36~48 h内形成褪绿斑,而低浓度的毒素(< 2.5 mg/ml)接种后随时间延长在接点无任何可见变化。依文斯蓝(Evans blue)染色发现,毒素和JXOⅢ分别在接种后0.5 h和6 h内细胞大量死亡。叶圆片法测定表明,接种毒素后2 h内烟草细胞膜透性迅速上升,接种JXOⅢ后9 h细胞膜透性开始明显上升。毒素处理的烟草叶片中LOX、POX、SOD、CAT活性水平较对照降低,而JXOⅢ处理中上述酶活性较对照有不同程度的增加。蛋白质合成抑制剂环己酰亚胺,RNA合成抑制剂放线菌素D和钙离子通道阻断剂氯化镧分别以7.1×10-5 M、1×10-4 M和1×10-3 M浓度有效地抑制JXOⅢ在烟草上形成坏死反应,这些抑制剂对毒素在烟草上的坏死反应则没有影响。
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| 关 键 词： | 白叶枯病菌 毒素 坏死 过敏反应 |
THE MECHANISM OF TOBACCO LEAF NECROSIS CAUSED BY Xanthomonas oryzae pv.oryzae AND ITS TOXIN |
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| Song Congfeng,Pan Xiaomei,Yang Yue,Wang Jinsheng.THE MECHANISM OF TOBACCO LEAF NECROSIS CAUSED BY Xanthomonas oryzae pv.oryzae AND ITS TOXIN[J].Acta Phytopathologica Sinica,1999,29(1):58-63. |
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| Authors: | Song Congfeng Pan Xiaomei Yang Yue Wang Jinsheng |
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| Institution: | The Key Lab. of Monitoring and Management of Plant Disease and Insects of Chinese Ministry of Agriculture, Dept. of Plant Protection, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | Both bacterial cells of Xanthomonas oryzae pv.oryzae (JXOⅢ) and its toxin caused rapid necrosis reaction at infiltrated sites on tobacco leaf.The time needed to initiate the reaction is 0.5 h for toxin and 8 h for JXOⅢ after infiltration.Infiltration with low concentration of JXOⅢ (<107cfu/ml)caused chlorosis at inoculation site 48 h later. While,inoculation with low concentration of toxin (<25mg/ml)gave no macroscopic changes.
Treatment with JXOⅢ and its toxin caused rapid cell death of tobacco leaf at 6 h and 0.5 h after inoculation respectively.Results of tobacco leaf discs' analysis shows that treatment with toxin rapidly increased membrane permeability. The enzyme activity levels of LOX,POX,CAT and SOD were greatly reduced compared with untreated control.While,treatment with JXOⅢ increased the levels of the above enzyme activities.Treatments with eucaryotic metabolic inhibitors,such as,cycloheximide,actinomycin D and lanthanum chloride prevented necrosis reaction on tobacco leaves by JXOⅢ, but not toxin.The results suggest that although JXOⅢ caused cell death by inducing active defense response of plant, its toxin caused cell death by direct to xicity on cell. |
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| Keywords: | Xanthomonas oryzae pv oryzae toxin necrosis HR |
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