| 水稻上三种条斑病细菌DNA的多态性初析 |
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| 引用本文： | 姬广海,孔繁明,何礼远.水稻上三种条斑病细菌DNA的多态性初析[J].植物病理学报,1999,29(2):120-125. |
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| 作者姓名： | 姬广海 孔繁明 何礼远 |
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| 作者单位： | 1 南京农业大学植保系, 南京 210095;2 中国农科院植保所, 北京 100094 |
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| 基金项目： | 中国农科院植保所国家重点实验室开放基金 |
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| 摘 要： | 试用40个引物对我国水稻条斑病菌、"稻短条斑病菌"和李氏禾条斑病菌等14个代表菌株进行RAPD分析,其中11个引物的扩增产物表现明显的多态性,共扩增出158条谱带,多态性为89.74%。聚类分析结果显示14个菌株可区分为3个类群,第1群包括LLS2、LLS3、LLS4、RS05、R1008、TAS和TAX;来自不同稻区水稻条斑病菌的群体结构差异明显,分属于第2群(如RS-Hai等)和第3群(如RS105等)。菌株DNA-RAPD指纹分析和致病性测定结果证明:李氏禾条斑病菌、"稻短条斑病菌"在水稻和李氏禾上不仅可相互侵染,而且遗传背景的相似性较高,确认是同一种病菌,其与小麦黑颖病菌亲缘关系较近,但与水稻细菌性条斑病菌具有明显差异。
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| 关 键 词： | “稻短条斑病菌” 水稻条斑病菌 李氏禾条斑病菌 RAPD |
| 修稿时间： | :1998-03-2 |
PRELIMINARY ANALYSIS OF DNA POLYMORPHISM OF Xanthomonas oryzae pv. oryzicola AND Xanthomonas leersiae BY USING RAPD |
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| Ji Guanghai,Kong Fanming,Shen Xiuping,Xu Zhigang.PRELIMINARY ANALYSIS OF DNA POLYMORPHISM OF Xanthomonas oryzae pv. oryzicola AND Xanthomonas leersiae BY USING RAPD[J].Acta Phytopathologica Sinica,1999,29(2):120-125. |
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| Authors: | Ji Guanghai Kong Fanming Shen Xiuping Xu Zhigang |
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| Institution: | 1 Dept of Plant Protection, Nanjing Agric Univ., Nanjing 210095;2 Institute of Plant Protection, Chinese Aca. of Agric. Sciences, Beijing 100094 |
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| Abstract: | Genetic diversity of 14 pathogenic bacteria isolates of the ‘rice short streak’ and the rice and leersiae bacterial leaf streak were assessed by RAPD with 40 primers.Eleven primers revealed reproducible polymorphism and a total of 158 bands were scored.The frequency of genetic diversity was 89.74%.Results from comparative resarch of cluster analysis base on the RAPD bands of 14 isolates and their pathogenicity on rice suggested that the relationship between LLS2,LLS3,LLS4,RS05,R1008,TAS,and TAX were very close,belonging to the same group (I).Highest similarity was found between TAS and TAX.High genetic diversity was observed among isolates derived from rice bacterial leaf streak,belonging to two different groups (Ⅱ,Ⅲ).Results indicated that isolates LLS2,LLS3,LLS4,R1008,RS05 are closer to the isolates derived from rice (TAS,TAX)than to the others. |
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| Keywords: | “rice short streak” leaf streak Xanthomonas oryzae pv oryzicola Xanthomonas leersiae RAPD |
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