| 小麦黄色花叶病毒不同分离物外壳蛋白(CP)基因序列的比较分析 |
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| 引用本文： | 韩成贵,李大伟,于嘉林.小麦黄色花叶病毒不同分离物外壳蛋白(CP)基因序列的比较分析[J].植物病理学报,1999,29(3):216-220. |
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| 作者姓名： | 韩成贵 李大伟 于嘉林 |
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| 作者单位： | 中国农业大学农业生物技术国家重点实验室和植物病理学系 北京 100094 |
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| 基金项目： | “863”计划课题，国家自然科学基金 |
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| 摘 要： | 核苷酸序列分析结果表明,小麦黄色花叶病毒(W YMV)不同分离物的外壳蛋白基因存在一定的差异。邓州分离物CP基因在其31~33nt处均缺失了3个核苷酸,其余分离物与潢川分离物及日本分离物长度一致,均为882nt。不同分离物CP基因核苷酸序列同源性为97.3%~98.9%,由此推导的氨基酸序列同源性为97.6%~99.3%,外壳蛋白N末端的110个氨基酸和C末端的55个氨基酸在各个分离物间是高度保守的。潢川分离物有5个氨基酸与其它5个分离物明显不同。WYMV不同分离物外壳蛋白序列分析结果进一步确认了WYMV与WSSMV为Bymovirus属的2种不同病毒。
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| 关 键 词： | 小麦黄色花叶病毒 分离物 外壳蛋白基因 序列比较 |
SEQUENCE COMPARISON OF THE GENE FOR COAT PROTEIN FROM DIFFERENT ISOLATES OF WHEAT YELLOW MOSAIC VIRUS |
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| Han Chenggui,Li Dawei,Yu Jialin,Xing Yiming,Zhu Kun,Tian Zhaofeng,Cai Zhunan,Liu Yi.SEQUENCE COMPARISON OF THE GENE FOR COAT PROTEIN FROM DIFFERENT ISOLATES OF WHEAT YELLOW MOSAIC VIRUS[J].Acta Phytopathologica Sinica,1999,29(3):216-220. |
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| Authors: | Han Chenggui Li Dawei Yu Jialin Xing Yiming Zhu Kun Tian Zhaofeng Cai Zhunan Liu Yi |
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| Institution: | National Laboratory for Agrobiotechnology and Department of Plant Pathology, China Agricultural University, Beijing 100094 |
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| Abstract: | The sequence analysis revealed that there were some nucleotide differences in CP genes of wheat yellow mosaic virus (WYMV) from Dengzhou city in Henan, Yangzhou city in Jiangsu, Yaan city in Sichuan, Zhouzhi county in Shaanxi province of China. Comparing with the Huangchuan isolate and Japanese isolate, the CP gene of Dengzhou isolate had three nucleotides deletion (31-33nt) at its 5'end, the rest isolates had the same length CP gene,containing 882 nucleotides. The isolates of WYMV exhibited nucleotide sequence homologies ran ging from 97.3% to 98.9% and shared deduced amino acid sequence homologies of 97.6% to 99.3%,110 amino acid residues of the N terminal sequences and 55 amino acid residues of C terminal sequences in CP of different WYMV isolates were extremely conservative. Huangchuan isolate had 5 amino acid residues obviously different from the rest 5 isolates. These sequence data on WYMV CP gene from different isolates further indicate that WYMV and wheat spindle streak mosaic virus (WSSMV) are two distinct virus species within the genus Bymovirus. |
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| Keywords: | wheat yellow mosaic virus isolates CP gene sequence comparison |
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